LOCUS V00083 3273 bp DNA circular SYN 17-MAY-1995
DEFINITION Artificial cloning vehicle pBR327, derived from pBR322. The
sequence was not resequenced but deduced from the pBR322 sequence.
Contains the reading frames for ampicillin resistance (Apr) and
tetracycline resistance (Tcr) and an origin of replication.
ACCESSION V00083 J02549
VERSION V00083.1
KEYWORDS circular; cloning vector; drug resistance gene; origin of
replication; plasmid.
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3273)
AUTHORS Soberon X., Covarrubias L., Bolivar F.
TITLE Construction and characterization of new cloning vehicles. IV.
Deletion derivatives of pBR322 and pBR325
JOURNAL Gene 9(3-4), 287-305(1980).
PUBMED 6248430
FEATURES Location/Qualifiers
source 1..3273
/organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
CDS 259..1275
/transl_table=11
/note="tetracycline resistance"
/protein_id="CAA23425.1"
/translation="MRTRSRSTVRPLWPPPSPARFATWSHYRLRDHGDHTRPVDPLRR
THRGRHHRRHRCGCWRLYRRHHRWGRSGSPLRAHERLFRRGYGGRPVAGGLLGAISLH
APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV
FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE
KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG
QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR
ATST"
CDS complement(2205..3065)
/transl_table=11
/note="ampicillin resistance"
/protein_id="CAA23426.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 721 a 911 c 835 g 806 t
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggcccgt ggccggggga
541 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
601 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
661 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
721 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
781 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
841 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
901 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
961 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
1021 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
1081 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
1141 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
1201 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
1261 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
1321 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
1381 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg
1441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
1501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
1561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1621 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
1741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
1801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
1861 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
1921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
1981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
2461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
2521 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
2581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
2821 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
2881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
2941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
3121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
3181 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
3241 aggcgtatca cgaggccctt tcgtcttcaa gaa
//