LOCUS CAA23426.1 286 aa PRT SYN 17-MAY-1995
DEFINITION synthetic construct protein ( Artificial cloning vehicle
pBR327, derived from pBR322. The sequence was not resequenced
but deduced from the pBR322 sequence. Contains the reading
frames for ampicillin resistance (Apr) and tetracycline
resistance (Tcr) and an origin of replication. ).
ACCESSION V00083-2
PROTEIN_ID CAA23426.1
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3273)
AUTHORS Soberon X., Covarrubias L., Bolivar F.
TITLE Construction and characterization of new cloning vehicles. IV.
Deletion derivatives of pBR322 and pBR325
JOURNAL Gene 9(3-4), 287-305(1980).
PUBMED 6248430
FEATURES Qualifiers
source /organism="synthetic construct"
/mol_type="other DNA"
/db_xref="taxon:32630"
protein /transl_table=11
/note="ampicillin resistance"
BEGIN
1 MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP
61 EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL
121 CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM
181 PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS
241 RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
//