LOCUS AY143338 9266 bp DNA linear BCT 02-APR-2003
DEFINITION Pseudomonas putida mandelate racemase (mdlA), S-mandelate
dehydrogenase (mdlB), benzoylformate decarboxylase (mdlC), putative
regulatory protein MdlX (mdlX), mandelamide hydrolase (mdlY), and
benzaldehyde dehydrogenase (mdlD) genes, complete cds.
ACCESSION AY143338 J05293
VERSION AY143338.1
KEYWORDS .
SOURCE Pseudomonas putida
ORGANISM Pseudomonas putida
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
REFERENCE 1 (bases 1 to 9266)
AUTHORS Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
Kenyon,G.L.
TITLE Mandelate pathway of Pseudomonas putida: sequence relationships
involving mandelate racemase, (S)-mandelate dehydrogenase, and
benzoylformate decarboxylase and expression of benzoylformate
decarboxylase in Escherichia coli
JOURNAL Biochemistry 29 (42), 9856-9862 (1990)
PUBMED 2271624
REFERENCE 2 (bases 1 to 9266)
AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S.,
Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D.
TITLE The crystal structure of benzoylformate decarboxylase at 1.6 A
resolution: diversity of catalytic residues in thiamin
diphosphate-dependent enzymes
JOURNAL Biochemistry 37 (28), 9918-9930 (1998)
PUBMED 9665697
REFERENCE 3 (bases 1 to 9266)
AUTHORS McLeish,M.J., Kneen,M.M., Gopalakrishna,K.N., Koo,C.W.,
Babbitt,P.C., Gerlt,J.A. and Kenyon,G.L.
TITLE Identification and characterization of a mandelamide hydrolase and
an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas
putida ATCC 12633
JOURNAL J. Bacteriol. 185 (8), 2451-2456 (2003)
PUBMED 12670968
REFERENCE 4 (bases 1 to 9266)
AUTHORS Tsou,A.Y., Ransom,S., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
Kenyon,G.L.
TITLE Direct Submission
JOURNAL Submitted (27-AUG-1990) Department of Chemistry and Biochemistry,
University of Maryland, College Park, MD 20742, USA
REFERENCE 5 (bases 1 to 9266)
AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S.,
Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D.
TITLE Direct Submission
JOURNAL Submitted (29-APR-1998) Purdue University, West Lafayette, IN
47907-1392, USA
REMARK Nucleotide and amino acid sequence updated by submitter
REFERENCE 6 (bases 1 to 9266)
AUTHORS McLeish,M.J., Kneen,M.M., Koo,C.C., Babbitt,P.C., Tsou,A.Y.,
Schafer,S.L., Gerlt,J.A. and Kenyon,G.L.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2002) College of Pharmacy, University of
Michigan, 428 Church St., Ann Arbor, MI 48109, USA
REMARK Nucleotide and amino acid sequence updated by submitter
COMMENT On Jan 15, 2003 this sequence version replaced J05293.1.
FEATURES Location/Qualifiers
source 1..9266
/organism="Pseudomonas putida"
/mol_type="genomic DNA"
/strain="ATCC 12633"
/type_material="type strain of Pseudomonas putida"
/db_xref="ATCC:12633"
/db_xref="taxon:303"
gene complement(594..1673)
/gene="mdlA"
CDS complement(594..1673)
/gene="mdlA"
/EC_number="5.1.2.2"
/codon_start=1
/transl_table=11
/product="mandelate racemase"
/protein_id="AAC15504.1"
/translation="MSEVLITGLRTRAVNVPLAYPVHTAVGTVGTAPLVLIDLATSAG
VVGHSYLFAYTPVALKSLKQLLDDMAAMIVNEPLAPVSLEAMLAKRFCLAGYTGLIRM
AAAGIDMAAWDALGKVHETPLVKLLGANARPVQAYDSHSLDGVKLATERAVTAAELGF
RAVKTKIGYPALDQDLAVVRSIRQAVGDDFGIMVDYNQSLDVPAAIKRSQALQQEGVT
WIEEPTLQHDYEGHQRIQSKLNVPVQMGENWLGPEEMFKALSIGACRLAMPDAMKIGG
VTGWIRASALAQQFGIPMSSHLFQEISAHLLAATPTAHWLERLDLAGSVIEPTLTFEG
GNAVIPDLPGVGIIWREKEIGKYLV"
gene complement(1670..2851)
/gene="mdlB"
CDS complement(1670..2851)
/gene="mdlB"
/codon_start=1
/transl_table=11
/product="S-mandelate dehydrogenase"
/protein_id="AAC15503.1"
/translation="MSQNLFNVEDYRKLRQKRLPKMVYDYLEGGAEDEYGVKHNRDVF
QQWRFKPKRLVDVSRRSLQAEVLGKRQSMPLLIGPTGLNGALWPKGDLALARAATKAG
IPFVLSTASNMSIEDLARQCDGDLWFQLYVIHREIAQGMVLKALHTGYTTLVLTTDVA
VNGYRERDLHNRFKIPMSYSAKVVLDGCLHPRWSLDFVRHGMPQLANFVSSQTSSLEM
QAALMSRQMDASFNWEALRWLRDLWPHKLLVKGLLSAEDADRCIAEGADGVILSNHGG
RQLDCAISPMEVLAQSVAKTGKPVLIDSGFRRGSDIVKALALGAEAVLLGRATLYGLA
ARGETGVDEVLTLLKADIDRTLAQIGCPDITSLSPDYLQNEGVTNTAPVDHLIGKGTH
A"
gene complement(2860..4446)
/gene="mdlC"
CDS complement(2860..4446)
/gene="mdlC"
/EC_number="4.1.1.7"
/codon_start=1
/transl_table=11
/product="benzoylformate decarboxylase"
/protein_id="AAC15502.1"
/translation="MASVHGTTYELLRRQGIDTVFGNPGSNELPFLKDFPEDFRYILA
LQEACVVGIADGYAQASRKPAFINLHSAAGTGNAMGALSNAWNSHSPLIVTAGQQTRA
MIGVEALLTNVDAANLPRPLVKWSYEPASAAEVPHAMSRAIHMASMAPQGPVYLSVPY
DDWDKDADPQSHHLFDRHVSSSVRLNDQDLDILVKALNSASNPAIVLGPDVDAANANA
DCVMLAERLKAPVWVAPSAPRCPFPTRHPCFRGLMPAGIAAISQLLEGHDVVLVIGAP
VFRYHQYDPGQYLKPGTRLISVTCDPLEAARAPMGDAIVADIGAMASALANLVEESSR
QLPTAAPEPAKVDQDAGRLHPETVFDTLNDMAPENAIYLNESTSTTAQMWQRLNMRNP
GSYYFCAAGGLGFALPAAIGVQLAEPERQVIAVIGDGSANYSISALWTAAQYNIPTIF
VIMNNGTYGALRWFAGVLEAENVPGLDVPGIDFRALAKGYGVQALKADNLEQLKGSLQ
EALSAKGPVLIEVSTVSPVK"
gene 4598..5299
/gene="mdlX"
CDS 4598..5299
/gene="mdlX"
/codon_start=1
/transl_table=11
/product="putative regulatory protein MdlX"
/protein_id="AAO23018.1"
/translation="MATVLRGTDMDQLAELVWLLAQFQGKPNARAAILQAVAAFLDAE
FVASFVWDKSEGRSIDPLAFNMESQFIEAYQKHFYQVDLVTPIMREVRAPARVDCHVP
RSAMLKSEFYNDFLQPADMEHGLNVFFFDGDRDIGDLRVWRSKGSKPFSEREERLLST
LQPYVQKAFSPAPTLSQRLSLREAEVAELVAQGASDKEVARRLDIAFSTVRTHLKNAM
AKLECQNRTQLARCL"
gene 5403..6926
/gene="mdlY"
CDS 5403..6926
/gene="mdlY"
/note="amidase"
/codon_start=1
/transl_table=11
/product="mandelamide hydrolase"
/protein_id="AAO23019.1"
/translation="MRHPVDMPEKVGTDAKRLFAQPEHLWELTLTEASALVRHRRITS
RQLVEAWLSRIADFSELNAFISVDAAAALKQADSYDHYLEAGGDPLPLGGVPIAVKDN
IQVVGFANTAGTPALSKFFPTCNARVIEPLLKAGAIVVGKTNMHELAFGTSGYNTAYH
IPGVIGVRNAFDHSCIAGGSSSGSGTAVGALLIPAALGTDTGGSVRQPGAVNGCVGFR
PTVGRYPVDGITPISPTRDTPGPIARSVEDIVLLDSIITGALPAEVPAAESIRLGVVD
QLWADLSEPVRKLTEDALRKLEQQGVQIVRVSMSEIFEMSHAVSMPLALHECRSALTE
YLSANETGVSFDELVAGISSPDVRTIFEDYILPGRLGELEGQSVDLEQAYATAMKDAR
PKLIQSFEFLFKEHQLDAIIHPTTPDLAIKSNPAATSFEAFARMIRNADPASNAGMPG
ISLPAGLSQQEGLPVGIEIEGLPGSDARLLSIANFIESILGRGPTPTRSGVESKISM"
gene 6994..8304
/gene="mdlD"
CDS 6994..8304
/gene="mdlD"
/note="NAD(P)-dependent aldehyde dehydrogenase"
/codon_start=1
/transl_table=11
/product="benzaldehyde dehydrogenase"
/protein_id="AAO23020.1"
/translation="MNYLSPAKIDSLFSAQKAYFATRATADVGFRKQSLERLKEAVIN
NKEALYSALAEDLGKPKDVVDLAEIGAVLHEIDFALAHLDEWVAPVSVPSPDIIAPSE
CYVVQEPYGVTYIIGPFNYPVNLTLTPLIGAIIGGNTCIIKPSETTPETSAVIEKIIA
EAFAPEYVAVIQGGRDENSHLLSLPFDFIFFTGSPNVGKVVMQAAAKHLTPVVLELGG
KCPLIVLPDADLDQTVNQLMFGKFINSGQTCIAPDYLYVHYSVKDALLERLVERVKTE
LPEINSTGKLVTERQVQRLVSLLEATQGQVLVGSQADVSKRALSATVVDGVEWNDPLM
SEELFGPILPVLEFDSVRTAIDQVNKHHPKPLAVYVFGKDMDVAKGIINQIQSGDAQV
NGVMLHAFSPYLPFGGIGASGMGEYHGHFSYLTFTHKKSVRIVP"
BASE COUNT 2078 a 2371 c 2561 g 2256 t
ORIGIN
1 gagctccagc gctggtgtct gccagccgaa ggcctacgtt ttgaggataa agtatacaag
61 ctaatcgacc tggaagggat tgcgcagctg ttgagccttg tctgtaacga gattgaaagt
121 gagaacatat tcgtgcggtt caagagcatt acgttcaaat tataattatc aaagcactta
181 cttctttttt gactaacctg gatgtaaagg gggagggagt gcagagtcaa gttttgcgca
241 aggtgctgca atatattgag gataatttga agcaagattt gacacctgaa acgttggctc
301 gttatgctaa cgtaagtgtg cgcagcctct acgttctctt cgagcagcac atgggcgagc
361 caccccgtcg gtattttgtc agcaggaaac tagatcggat acgtgcggct ttgttgaatg
421 ctgacggtcg aaaaaagagt gtaactgaac tcgcgatgga ttacggtttt acgcatttgg
481 gccgtttctc ggctgaatat aaagccaggt tttcagagct accttccgaa acgctaaagc
541 atggaaagtc tgtataagaa aaggcccgca taagcgggcc tttttatttt gcattacacc
601 agatatttcc cgatttcttt ctcgcgccag atgatcccaa cgccagggag atccggaatc
661 acagcatttc cgccctcaaa tgtaagagta ggctcgatga cgctgccggc gagatccaaa
721 cgctccagcc aatgcgcagt tggggtggcg gctagcaggt gagcgctgat ttcttggaac
781 aggtggctgg acattggaat accgaattgt tgtgcgagtg cactggctcg gatccagccc
841 gtcacgccac cgatcttcat tgcatctggc atagccaacc ggcatgcacc gatgctcaat
901 gctttgaaca tctcctcagg gccgagccag ttctcaccca tctggacggg cacattgagc
961 ttgctttgaa tgcgctgatg gccttcgtaa tcgtgttgaa gcgtcggctc ttcaatccag
1021 gtgacgccct cttgctgcag cgcctggctg cgtttgattg cggctggtac atccaaactc
1081 tggttgtagt cgaccatgat gccgaagtcg tcacctactg cttggcgtat gctgcgcacg
1141 actgccagat cttgatccaa tgccggatag ccgatcttgg tcttaaccgc ccggaatccg
1201 agttctgctg cggtcacagc gcgctcagtc gcgagtttga ctccatccaa gctgtggctg
1261 tcataagcct gaactggtcg agcgttcgcg ccgaggagtt ttactagtgg cgtttcgtga
1321 accttgccca gcgcatccca agctgccata tcgatgccag cagcggccat tcggatcaat
1381 cccgtataac ctgccaggca gaagcgtttt gcgagcatgg cttccaagct aaccggtgcc
1441 aatggttcgt tgacgatcat ggctgccatg tcgtccagga gctgcttcag cgacttcaac
1501 gcaacggggg tgtatgcgaa caggtaggaa tggcctacca cgccggcact ggtggccagg
1561 tcgatcagaa caagaggcgc tgtgccaaca gttccaacag cggtgtgaac ggggtaggcc
1621 aatgggacat tgacggcccg cgtcctcagg ccggtaatca gtacttcact catgcgtgtg
1681 ttcctttacc aatgaggtga tcgactggag cggtgttagt cactccctcg ttttggaggt
1741 aatcaggaga aagggaggtg atgtcagggc atccaatctg ggcaagggtg cggtcgatat
1801 ccgcttttag gagggttagc acctcgtcaa cacccgtttc acctcgtgct gcaaggccat
1861 acaaagttgc acgacccagg agtacagcct cagcacctag cgcaagtgct ttaacgatgt
1921 ccgaaccccg tcggaagccg ctatcgataa gcactggttt tccagttttc gctaccgatt
1981 gagccaaaac ttccattggc gatatcgcgc aatcgagttg gcgaccgccg tggtttgata
2041 ggattacgcc gtctgcacct tcagcgatgc accgatcggc gtcctcagca ctgagcaacc
2101 cctttacgag gagtttgtgc ggccagaggt cacgcagcca tctcaatgcc tcccagttga
2161 aactggcatc catttggcgg ctcatcaatg ctgcctgcat ttctaagcta gacgtttgac
2221 tgctgacgaa attggccagt tgcggcatgc cgtgtcgcac gaagtcgagc gaccagcgcg
2281 gatgcaggca tccgtccagc accacctttg cggagtagct cattggtatc ttgaatcggt
2341 tatgcaggtc gcgctcgcga tagccgttaa ccgccacatc cgtagtaagc accagtgtcg
2401 tgtaaccagt gtgcagggct ttgagcacca tcccctgcgc aatctctcgg tggatcacat
2461 agagctggaa ccacagatcg ccatcacact gacgtgcgag gtcttcaatg gacatgttgg
2521 aggcggtcga cagcacgaac gggattccgg ccttggttgc tgctcgagct aaagcgagat
2581 cccccttagg ccacagcgca ccgttcagcc cagtaggccc aatcaagaga ggcatcgact
2641 gcctctttcc aagtacttcc gcttggaggc tgcggcggct gacgtctact agccgcttcg
2701 gtttgaatcg ccattgctgg aagacgtcgc ggttgtgttt caccccgtat tcgtcttcag
2761 ccccaccttc cagatagtcg tagaccatct tcggcaagcg cttttgccga agcttgcgat
2821 agtcctcaac gttaaagaga ttctggctca tggcttacct cacttcaccg ggcttacggt
2881 gcttacttcg ataagtaccg ggcctttggc agaaagcgct tcttgtagcg aacccttgag
2941 ctgctcaagg ttgtcggctt tcagcgcttg gacaccatag cccttggcga gtgcgcggaa
3001 gtcgatccct ggcacatcca gcccaggaac gttttctgct tcgagaacgc cggcaaacca
3061 tcgcaacgca ccgtaggtgc cgttgttcat gatcacgaag atagtgggga tgttgtactg
3121 agctgcagtc cacaacgcac taatgctgta gttcgccgat ccgtcgccaa tgacggcgat
3181 gacttgtcgc tcgggttctg cgagttgaac gccaattgct gcaggcaggg cgaagcccag
3241 tccgccagct gcacagaagt agtagctacc agggttgcgc atgttcaggc gctgccacat
3301 ttgggcggtc gttgaagtcg actcgttcag gtaaatcgca ttctccgggg ccatgtcgtt
3361 cagtgtgtcg aacactgtct ctgggtgaag tcggccagcg tcttggtcaa ccttcgcggg
3421 ttccggagct gcagttggga gctggcggct gctctcttca accaagttgg caagagcgct
3481 agccatcgca ccaatgtctg ccacgatcgc atcgcccatt ggcgcgcgtg cagcttcgag
3541 cgggtcgcag gtcaccgaaa tcaatcgcgt gccaggtttg agatattgac ctgggtcgta
3601 ttggtggtaa cggaacactg gagcgccgat taccaaaacc acatcgtgac cttcgagcag
3661 ctgagaaatc gctgcgatgc cagctggcat caatccacgg aagcaaggat gacgggtagg
3721 gaatgggcag cgtggagcgg atggcgcaac ccaaaccgga gctttgaggc gttcggccaa
3781 catgacgcag tctgcgttcg catttgctgc gtcgacgtcc gggcccagga cgatcgccgg
3841 gttggatgcg ctgttgagag ctttcaccag aatatcgaga tcctggtcgt tcaggcgtac
3901 tgatgaactg acatggcgat caaaaaggtg gtgggactga ggatcagcat ccttatccca
3961 atcgtcatat ggcaccgaaa gatagacagg gccttgtggc gccatgcttg ccatatggat
4021 agccctgctc atcgcatgag ggacttctgc tgcgcttgcg ggctcgtagc tccatttgac
4081 aagtggtcgt ggcaggttgg cggcatcgac gttggtcagc agagcttcaa cgccaatcat
4141 cgccctggtc tgctggccgg cagtgacgat cagcggggaa tgtgagttcc aggcgttact
4201 gagtgcaccc atagcattgc cggtaccagc agcagaatgc aggttaatga aagccggctt
4261 ccgactggct tgcgcatagc cgtctgcaat gcccaccaca cacgcttcct gcaaagccag
4321 gatgtatcga aagtcctctg gaaagtcctt caaaaacggg agctcgttcg agccaggatt
4381 gccgaagacc gtatcgatgc cttgacgtcg caagagttcg tatgtggtgc cgtgtaccga
4441 agccatgatt tttatctctt accaaatggt gaatcccacg ataaaatcga gacctaaata
4501 gatcaaatag atagattcta aaataatata tagatcggtt ctattttgat gcttttaccc
4561 ttctgtgacg ctttgacagc attttcggag gtaggcaatg gcaacagtgc tgcgtggaac
4621 cgatatggat cagcttgctg agctggtctg gcttcttgct cagttccagg gaaaaccaaa
4681 cgctcgggct gcgattttgc aagcggtagc ggcctttctc gacgcggagt tcgttgcttc
4741 attcgtatgg gacaaaagtg aggggcgatc catcgacccg ctggctttca atatggagag
4801 ccagttcata gaggcttatc agaagcactt ttaccaggtc gatttggtaa ccccgatcat
4861 gcgagaggtg agggcgccgg cgcgagtcga ctgccatgtg ccacgttcag caatgctcaa
4921 gagcgagttc tacaacgact tccttcagcc ggcagatatg gagcatggcc tcaacgtctt
4981 tttcttcgac ggtgatcggg acatcggcga tctcagggta tggaggagca aggggagcaa
5041 acccttcagc gagagagaag aacgtctgct atcgaccctc cagccatatg ttcagaaagc
5101 ttttagcccg gcgccgacac tttcccaacg attgtcgttg agagaagctg aggtggccga
5161 acttgtggct cagggggcca gcgataaaga ggtcgcccgc cgcttggata tagcgttcag
5221 cacggtacgc actcatctga agaacgccat ggcgaagctt gagtgtcaga accgcactca
5281 gctagcccgc tgcttatgaa atgtttctgg caatcctcaa atttggggag acctattgcc
5341 gcaggcccac attaccgttg tgtgaaccac agcatcgcca gtgcggctag gagaggtaaa
5401 aaatgcgcca cccagtagat atgcctgaaa aggtcggtac cgacgcaaag cgtctgttcg
5461 cacaacccga gcatctttgg gagctcaccc tcaccgaagc gtctgccttg gttcggcatc
5521 gacggatcac aagccgccag cttgtggaag cgtggctttc tcggatcgcg gacttctctg
5581 agctgaacgc atttatcagc gttgatgcag cggccgccct gaagcaagca gattcgtatg
5641 atcactacct tgaggcgggt ggtgatccgc tgccattggg cggtgtaccg atcgcggtaa
5701 aggacaacat ccaggtggtt ggcttcgcga acacggccgg gactccagcg ctgtccaaat
5761 tctttccaac gtgtaacgcc cgagttattg agccgttgct caaagccggt gcaatcgttg
5821 ttgggaagac caacatgcat gagctagcgt ttggcacctc gggctataac accgcgtacc
5881 acatccctgg cgttataggt gttcgtaacg catttgacca ctcatgcatc gcgggcgggt
5941 cgtcctctgg gagcggtact gctgtcggcg cacttttaat tcctgcagct ttgggtaccg
6001 atacaggtgg atctgttcga cagcctggtg ccgtgaatgg atgcgttggt tttcgaccaa
6061 cagttgggcg ttatccagtc gacggaatca caccgatttc cccaacgcgc gatacgccgg
6121 gcccaatcgc tcgctcggtc gaagacatag tgctgctgga ctcgatcatt accggggccc
6181 tgcctgcgga agttcctgct gccgagtcga ttcgactggg ggtagttgat caattgtggg
6241 ctgatctcag cgaaccggtt agaaagctga ctgaagatgc gctgagaaag cttgagcagc
6301 aaggtgtgca gattgtgagg gtttcgatgt ccgaaatctt cgagatgagt catgcagtca
6361 gtatgccgtt ggcattgcac gaatgcaggt cagccctaac ggaataccta tcggcaaacg
6421 agaccggtgt ttccttcgac gagctggtcg ctgggatcag cagcccagat gtccgaacca
6481 tctttgaaga ttacatcctg cctggaaggc tgggggagct ggaaggtcaa tctgtggatc
6541 ttgagcaggc ctacgccact gctatgaagg atgctcgtcc gaagctcatc cagagcttcg
6601 agtttctatt caaagagcat caactcgacg cgattatcca tcccaccacg cctgacctcg
6661 cgatcaagag caatcccgct gcgaccagtt tcgaggcctt cgcacgcatg attcgaaatg
6721 ccgacccggc cagtaatgct ggcatgcctg gtattagcct gcctgccggg ttgtctcaac
6781 aagaaggctt gccggtgggt attgagatcg aggggcttcc gggcagtgat gcgcgattgc
6841 tcagtatcgc aaatttcatc gagagcattt tggggcgtgg cccgactccg acgcgttcgg
6901 gggttgaaag caaaatttcc atgtagcact gccgatccca agtcggttac tacctatagc
6961 gcctgtaatc ccgaataaag aggaaatcca ttcatgaatt atctgtctcc ggctaagatt
7021 gactcgctgt tctccgctca gaaggcgtac tttgctaccc gagctacggc tgatgtgggc
7081 ttccgcaagc aatcgctaga aagactgaaa gaggcggtta tcaataacaa ggaagctctt
7141 tattccgcgc ttgcagagga cttgggcaag cccaaggatg tagtcgacct ggcagagatc
7201 ggtgcagtat tgcacgaaat tgattttgcg cttgctcacc ttgatgaatg ggtcgcgcca
7261 gtttcggttc cgtcgccaga cattattgcg ccttccgaat gttatgtagt ccaagagccg
7321 tacggtgtca cctatatcat cggccccttc aactacccag ttaacctgac tctcaccccg
7381 ctgattggtg cgataatcgg cggtaacact tgcatcatca agccatctga aaccactccg
7441 gaaacctcgg cggtaatcga gaagatcatt gctgaggcgt ttgctcctga atatgtcgcg
7501 gttatccagg gtggccgaga cgaaaatagt catttgctca gcctaccttt cgacttcatt
7561 tttttcactg gtagccccaa tgttggcaag gtggttatgc aggcggctgc taaacacctg
7621 actcctgtcg ttctagaact cggtggtaag tgcccactga ttgtcctgcc tgacgcggat
7681 ctcgatcaga cggtcaatca attgatgttc ggtaaattca taaacagtgg tcaaacctgc
7741 attgcaccgg attacctcta tgtgcattac agtgtcaaag atgcactttt ggagcgcctt
7801 gttgagcgcg tgaagaccga gctgcccgag atcaactcta ccggcaagct cgtcaccgag
7861 cgtcaggtgc agcgattggt ctccctgctg gaagcaacgc aagggcaggt gcttgtagga
7921 tctcaggcgg acgtaagcaa gcgcgccttg agtgctacgg tagtagatgg tgtggagtgg
7981 aatgaccctc tgatgtccga ggagcttttc ggccctattc tgcctgttct tgaatttgat
8041 agcgttcgga ctgcaattga tcaagtcaac aagcaccatc caaaaccttt ggccgtttat
8101 gtgttcggta aggatatgga tgtcgccaaa ggtatcatca accagattca gtctggtgat
8161 gcgcaggtga acggcgtaat gctccatgcg ttctcgccat acctcccgtt cgggggaatc
8221 ggtgcttctg gtatgggtga ataccatggg catttcagct acctgacctt tactcataaa
8281 aagtccgtgc ggattgtgcc gtaattatag atgcaagcgg cgctccggcg cgcttcttgt
8341 gctattggat ttcagatcaa cagcattgtc tggcctagtt tgttttcatt aacgaacgtc
8401 aggaggtagg atatgaccac gggcaaagac tttatcccaa ggcgatgcca ggacgaatct
8461 atcgcttgta gtcaagatca gaatatattg aaaagctatc catcaaatag tggagtgcac
8521 ctggaggcat tagatgcagg ctctctcgaa taattgaatt ttatcaagaa gatacgggaa
8581 ctcagcggct agctcttctg cttttggtga gatttctata atctgtcatc tgatctttct
8641 gagcctttgg gtctattaag tgttagtgtg agtaatgttg aagcgcctgc tgttttcaag
8701 attttgtatg tgcgcgccgt caacttggta gagtcgatgc tgatctcaat actgattaag
8761 gctatcgtaa ttcgtccagg tttgcgagcc gggtttaaaa tcagctcgtt ggagggtggg
8821 ttatcttcag gtatgaagaa atttcatcgg aggagagagg ggtggcagtt gatgttgcgt
8881 ccagtctgag cttgaagacc ttttgcaatt atgaatattt gttaattgag gtttttggtg
8941 cgcgacttga aatgttgaat ttcactgcta tagatcgaat tttcgcgaag cgtcacgacc
9001 tccttgtccg tcatgggagg tgtattgaag gccaatcagc gcaggttaca tcagaggaag
9061 tggagcgcgt gatagccaca cttcgcgact tcgcagtggc ttaatatcaa tatttatcac
9121 tcagtgtttg atcacaattc ctcggatatt tgagtaccgc tatctgaaag cctcaccctg
9181 tattgatcgt tcagcgtcag ggcagtttgg attcgctcca cgaaaccgga tacacccctg
9241 cacgaagcgg atagtgggcg gaattc
//