LOCUS       AAO23019.1               507 aa    PRT              BCT 02-APR-2003
DEFINITION  Pseudomonas putida mandelamide hydrolase protein.
ACCESSION   AY143338-5
PROTEIN_ID  AAO23019.1
SOURCE      Pseudomonas putida
  ORGANISM  Pseudomonas putida
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
            Kenyon,G.L.
  TITLE     Mandelate pathway of Pseudomonas putida: sequence relationships
            involving mandelate racemase, (S)-mandelate dehydrogenase, and
            benzoylformate decarboxylase and expression of benzoylformate
            decarboxylase in Escherichia coli
  JOURNAL   Biochemistry 29 (42), 9856-9862 (1990)
   PUBMED   2271624
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S.,
            Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D.
  TITLE     The crystal structure of benzoylformate decarboxylase at 1.6 A
            resolution: diversity of catalytic residues in thiamin
            diphosphate-dependent enzymes
  JOURNAL   Biochemistry 37 (28), 9918-9930 (1998)
   PUBMED   9665697
REFERENCE   3  (bases 1 to 9266)
  AUTHORS   McLeish,M.J., Kneen,M.M., Gopalakrishna,K.N., Koo,C.W.,
            Babbitt,P.C., Gerlt,J.A. and Kenyon,G.L.
  TITLE     Identification and characterization of a mandelamide hydrolase and
            an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas
            putida ATCC 12633
  JOURNAL   J. Bacteriol. 185 (8), 2451-2456 (2003)
   PUBMED   12670968
REFERENCE   4  (bases 1 to 9266)
  AUTHORS   Tsou,A.Y., Ransom,S., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and
            Kenyon,G.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-1990) Department of Chemistry and Biochemistry,
            University of Maryland, College Park, MD 20742, USA
REFERENCE   5  (bases 1 to 9266)
  AUTHORS   Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S.,
            Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-1998) Purdue University, West Lafayette, IN
            47907-1392, USA
  REMARK    Nucleotide and amino acid sequence updated by submitter
REFERENCE   6  (bases 1 to 9266)
  AUTHORS   McLeish,M.J., Kneen,M.M., Koo,C.C., Babbitt,P.C., Tsou,A.Y.,
            Schafer,S.L., Gerlt,J.A. and Kenyon,G.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2002) College of Pharmacy, University of
            Michigan, 428 Church St., Ann Arbor, MI 48109, USA
  REMARK    Nucleotide and amino acid sequence updated by submitter
COMMENT     On Jan 15, 2003 this sequence version replaced J05293.1.
FEATURES             Qualifiers
     source          /organism="Pseudomonas putida"
                     /mol_type="genomic DNA"
                     /strain="ATCC 12633"
                     /type_material="type strain of Pseudomonas putida"
                     /db_xref="ATCC:12633"
                     /db_xref="taxon:303"
     protein         /gene="mdlY"
                     /note="amidase"
                     /transl_table=11
BEGIN
        1 MRHPVDMPEK VGTDAKRLFA QPEHLWELTL TEASALVRHR RITSRQLVEA WLSRIADFSE
       61 LNAFISVDAA AALKQADSYD HYLEAGGDPL PLGGVPIAVK DNIQVVGFAN TAGTPALSKF
      121 FPTCNARVIE PLLKAGAIVV GKTNMHELAF GTSGYNTAYH IPGVIGVRNA FDHSCIAGGS
      181 SSGSGTAVGA LLIPAALGTD TGGSVRQPGA VNGCVGFRPT VGRYPVDGIT PISPTRDTPG
      241 PIARSVEDIV LLDSIITGAL PAEVPAAESI RLGVVDQLWA DLSEPVRKLT EDALRKLEQQ
      301 GVQIVRVSMS EIFEMSHAVS MPLALHECRS ALTEYLSANE TGVSFDELVA GISSPDVRTI
      361 FEDYILPGRL GELEGQSVDL EQAYATAMKD ARPKLIQSFE FLFKEHQLDA IIHPTTPDLA
      421 IKSNPAATSF EAFARMIRNA DPASNAGMPG ISLPAGLSQQ EGLPVGIEIE GLPGSDARLL
      481 SIANFIESIL GRGPTPTRSG VESKISM
//