LOCUS AAO23019.1 507 aa PRT BCT 02-APR-2003 DEFINITION Pseudomonas putida mandelamide hydrolase protein. ACCESSION AY143338-5 PROTEIN_ID AAO23019.1 SOURCE Pseudomonas putida ORGANISM Pseudomonas putida Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 9266) AUTHORS Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and Kenyon,G.L. TITLE Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli JOURNAL Biochemistry 29 (42), 9856-9862 (1990) PUBMED 2271624 REFERENCE 2 (bases 1 to 9266) AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S., Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D. TITLE The crystal structure of benzoylformate decarboxylase at 1.6 A resolution: diversity of catalytic residues in thiamin diphosphate-dependent enzymes JOURNAL Biochemistry 37 (28), 9918-9930 (1998) PUBMED 9665697 REFERENCE 3 (bases 1 to 9266) AUTHORS McLeish,M.J., Kneen,M.M., Gopalakrishna,K.N., Koo,C.W., Babbitt,P.C., Gerlt,J.A. and Kenyon,G.L. TITLE Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633 JOURNAL J. Bacteriol. 185 (8), 2451-2456 (2003) PUBMED 12670968 REFERENCE 4 (bases 1 to 9266) AUTHORS Tsou,A.Y., Ransom,S., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and Kenyon,G.L. TITLE Direct Submission JOURNAL Submitted (27-AUG-1990) Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA REFERENCE 5 (bases 1 to 9266) AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S., Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D. TITLE Direct Submission JOURNAL Submitted (29-APR-1998) Purdue University, West Lafayette, IN 47907-1392, USA REMARK Nucleotide and amino acid sequence updated by submitter REFERENCE 6 (bases 1 to 9266) AUTHORS McLeish,M.J., Kneen,M.M., Koo,C.C., Babbitt,P.C., Tsou,A.Y., Schafer,S.L., Gerlt,J.A. and Kenyon,G.L. TITLE Direct Submission JOURNAL Submitted (20-AUG-2002) College of Pharmacy, University of Michigan, 428 Church St., Ann Arbor, MI 48109, USA REMARK Nucleotide and amino acid sequence updated by submitter COMMENT On Jan 15, 2003 this sequence version replaced J05293.1. FEATURES Qualifiers source /organism="Pseudomonas putida" /mol_type="genomic DNA" /strain="ATCC 12633" /type_material="type strain of Pseudomonas putida" /db_xref="ATCC:12633" /db_xref="taxon:303" protein /gene="mdlY" /note="amidase" /transl_table=11 BEGIN 1 MRHPVDMPEK VGTDAKRLFA QPEHLWELTL TEASALVRHR RITSRQLVEA WLSRIADFSE 61 LNAFISVDAA AALKQADSYD HYLEAGGDPL PLGGVPIAVK DNIQVVGFAN TAGTPALSKF 121 FPTCNARVIE PLLKAGAIVV GKTNMHELAF GTSGYNTAYH IPGVIGVRNA FDHSCIAGGS 181 SSGSGTAVGA LLIPAALGTD TGGSVRQPGA VNGCVGFRPT VGRYPVDGIT PISPTRDTPG 241 PIARSVEDIV LLDSIITGAL PAEVPAAESI RLGVVDQLWA DLSEPVRKLT EDALRKLEQQ 301 GVQIVRVSMS EIFEMSHAVS MPLALHECRS ALTEYLSANE TGVSFDELVA GISSPDVRTI 361 FEDYILPGRL GELEGQSVDL EQAYATAMKD ARPKLIQSFE FLFKEHQLDA IIHPTTPDLA 421 IKSNPAATSF EAFARMIRNA DPASNAGMPG ISLPAGLSQQ EGLPVGIEIE GLPGSDARLL 481 SIANFIESIL GRGPTPTRSG VESKISM //