LOCUS AAC15504.1 359 aa PRT BCT 02-APR-2003 DEFINITION Pseudomonas putida mandelate racemase protein. ACCESSION AY143338-1 PROTEIN_ID AAC15504.1 SOURCE Pseudomonas putida ORGANISM Pseudomonas putida Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. REFERENCE 1 (bases 1 to 9266) AUTHORS Tsou,A.Y., Ransom,S.C., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and Kenyon,G.L. TITLE Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli JOURNAL Biochemistry 29 (42), 9856-9862 (1990) PUBMED 2271624 REFERENCE 2 (bases 1 to 9266) AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S., Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D. TITLE The crystal structure of benzoylformate decarboxylase at 1.6 A resolution: diversity of catalytic residues in thiamin diphosphate-dependent enzymes JOURNAL Biochemistry 37 (28), 9918-9930 (1998) PUBMED 9665697 REFERENCE 3 (bases 1 to 9266) AUTHORS McLeish,M.J., Kneen,M.M., Gopalakrishna,K.N., Koo,C.W., Babbitt,P.C., Gerlt,J.A. and Kenyon,G.L. TITLE Identification and characterization of a mandelamide hydrolase and an NAD(P)+-dependent benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633 JOURNAL J. Bacteriol. 185 (8), 2451-2456 (2003) PUBMED 12670968 REFERENCE 4 (bases 1 to 9266) AUTHORS Tsou,A.Y., Ransom,S., Gerlt,J.A., Buechter,D.D., Babbitt,P.C. and Kenyon,G.L. TITLE Direct Submission JOURNAL Submitted (27-AUG-1990) Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA REFERENCE 5 (bases 1 to 9266) AUTHORS Hasson,M.S., Muscate,A., McLeish,M.J., Polovnikova,L.S., Gerlt,J.A., Kenyon,G.L., Petsko,G.A. and Ringe,D. TITLE Direct Submission JOURNAL Submitted (29-APR-1998) Purdue University, West Lafayette, IN 47907-1392, USA REMARK Nucleotide and amino acid sequence updated by submitter REFERENCE 6 (bases 1 to 9266) AUTHORS McLeish,M.J., Kneen,M.M., Koo,C.C., Babbitt,P.C., Tsou,A.Y., Schafer,S.L., Gerlt,J.A. and Kenyon,G.L. TITLE Direct Submission JOURNAL Submitted (20-AUG-2002) College of Pharmacy, University of Michigan, 428 Church St., Ann Arbor, MI 48109, USA REMARK Nucleotide and amino acid sequence updated by submitter COMMENT On Jan 15, 2003 this sequence version replaced J05293.1. FEATURES Qualifiers source /organism="Pseudomonas putida" /mol_type="genomic DNA" /strain="ATCC 12633" /type_material="type strain of Pseudomonas putida" /db_xref="ATCC:12633" /db_xref="taxon:303" protein /gene="mdlA" /EC_number="5.1.2.2" /transl_table=11 BEGIN 1 MSEVLITGLR TRAVNVPLAY PVHTAVGTVG TAPLVLIDLA TSAGVVGHSY LFAYTPVALK 61 SLKQLLDDMA AMIVNEPLAP VSLEAMLAKR FCLAGYTGLI RMAAAGIDMA AWDALGKVHE 121 TPLVKLLGAN ARPVQAYDSH SLDGVKLATE RAVTAAELGF RAVKTKIGYP ALDQDLAVVR 181 SIRQAVGDDF GIMVDYNQSL DVPAAIKRSQ ALQQEGVTWI EEPTLQHDYE GHQRIQSKLN 241 VPVQMGENWL GPEEMFKALS IGACRLAMPD AMKIGGVTGW IRASALAQQF GIPMSSHLFQ 301 EISAHLLAAT PTAHWLERLD LAGSVIEPTL TFEGGNAVIP DLPGVGIIWR EKEIGKYLV //