LOCUS AJ000503 3525 bp mRNA linear HUM 14-NOV-2006
DEFINITION Homo sapiens mRNA for tyrosinase-related protein 2 (TRP-2).
ACCESSION AJ000503
VERSION AJ000503.1
KEYWORDS TRP-2; tyrosinase-related protein 2.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1
AUTHORS Lupetti R.
JOURNAL Submitted (18-JUL-1997) to the INSDC. Lupetti R., Experimental
Oncology D, Istituto Nazionale Tumori, via Venezian, 1, 20133
Milano, ITALY.
REMARK Revised by [4]
REFERENCE 2
AUTHORS Yokoyama K., Suzuki H., Yasumoto K., Tomita Y., Shibahara S.
TITLE Molecular cloning and functional analysis of a cDNA coding for
human DOPAchrome tautomerase/tyrosinase-related protein-2
JOURNAL Biochim. Biophys. Acta 1217(3), 317-321(1994).
PUBMED 8148378
REFERENCE 3
AUTHORS Sturm R.A., O'Sullivan B.J., Box N.F., Smith A.G., Smit S.E.,
Puttick E.R., Parsons P.G., Dunn I.S.
TITLE Chromosomal structure of the human TYRP1 and TYRP2 loci and
comparison of the tyrosinase-related protein gene family
JOURNAL Genomics 29(1), 24-34(1995).
PUBMED 8530077
REFERENCE 4 (bases 1 to 3525)
AUTHORS Lupetti R.
JOURNAL Submitted (10-JUL-1998) to the INSDC. Lupetti R., Experimental
Oncology D, Istituto Nazionale Tumori, via Venezian, 1, 20133
Milano, ITALY.
REFERENCE 5
AUTHORS Lupetti R., Pisarra P., Verrecchia A., Farina C., Nicolini G.,
Anichini A., Bordignon C., Sensi M.L., Parmiani G., Traversari C.
TITLE Translation of a retained intron in tyrosinase-related protein
(TRP) 2 mRNA generates a new cytotoxic T lymphocyte (CTL)-defined
and shared human melanoma antigen not expressed in normal cells of
the melanocytic lineage
JOURNAL J. Exp. Med. 188(6), 1005-1016(1998).
PUBMED 9743519
FEATURES Location/Qualifiers
source 1..3525
/db_xref="H-InvDB:HIT000243811"
/organism="Homo sapiens"
/chromosome="13"
/map="q3"
/isolate="patient 18732"
/mol_type="mRNA"
/dev_stage="adult"
/cell_line="Me18732"
/cell_type="melanoma"
/tissue_type="lymphonode metastasis"
/db_xref="taxon:9606"
5'UTR 1..399
/gene="TRP-2"
/function="DOPAchrome tautomerase"
/citation=[2]
CDS 400..1113
/codon_start=1
/gene="TRP-2"
/product="tyrosinase-related protein-2"
/function="DOPAchrome tautomerase"
/EC_number="5.3.3.12"
/note="This translation product contains a sequence (aa
200-237) derived from the retained intron 2 of the
partially spliced TRP-2 mRNA. Aminoacids 222-231 code for
a 10-mer which is a shared melanoma antigen recognized by
cytotoxic T lymphocytes."
/db_xref="GOA:O75767"
/db_xref="H-InvDB:HIT000243811.14"
/db_xref="InterPro:IPR008922"
/db_xref="UniProtKB/TrEMBL:O75767"
/experiment="experimental evidence, no additional details
recorded"
/protein_id="CAA04137.1"
/translation="MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRL
GAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFA
GYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITT
QHWVGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGGFFPWLKVYYYRFVIGLRV
WQWEVISCKLIKRATTRQP"
exon 400..694
/gene="TRP-2"
/number=1
exon 695..994
/gene="TRP-2"
/number=2
intron 995..3081
/gene="TRP-2"
/number=2
exon 3082..3182
/gene="TRP-2"
/number=3
exon 3183..3349
/gene="TRP-2"
/number=4
intron 3350..>3525
/gene="TRP-2"
/number=4
BASE COUNT 978 a 677 c 819 g 1051 t
ORIGIN
1 agctaaggag ggagggagag ggtttagaaa taccagcata ataagtagta tgactgggtg
61 ctctgtaaat taactcaatt agacaaagcc tgacttaacg ggggaagatg gtgagaagcg
121 ctaccctcat taaatttggt tgttagaggc gcttctaagg aaattaagtc tgttagttgt
181 ttgaatcaca taaaattgtg tgtgcacgtt catgtacaca tgtgcacaca tgtaacctct
241 gtgattcttg tgggtatttt tttaagaaga aaggaataga aagcaaagaa aaataaaaaa
301 tactgaaaag aaaagactga aagagtagaa gataaggaga aaagtacgac agagacaagg
361 aaagtaagag agagagagag ctctcccaat tataaagcca tgagccccct ttggtggggg
421 tttctgctca gttgcttggg ctgcaaaatc ctgccaggag cccagggtca gttcccccga
481 gtctgcatga cggtggacag cctagtgaac aaggagtgct gcccacgcct gggtgcagag
541 tcggccaatg tctgtggctc tcagcaaggc cgggggcagt gcacagaggt gcgagccgac
601 acaaggccct ggagtggtcc ctacatccta cgaaaccagg atgaccgtga gctgtggcca
661 agaaaattct tccaccggac ctgcaagtgc acaggaaact ttgccggcta taattgtgga
721 gactgcaagt ttggctggac cggtcccaac tgcgagcgga agaaaccacc agtgattcgg
781 cagaacatcc attccttgag tcctcaggaa agagagcagt tcttgggcgc cttagatctc
841 gcgaagaaga gagtacaccc cgactacgtg atcaccacac aacactgggt gggcctgctt
901 gggcccaatg gaacccagcc gcagtttgcc aactgcagtg tttatgattt ttttgtgtgg
961 ctccattatt attctgttag agatacatta ttaggtgggt tttttccttg gctgaaggta
1021 tattattaca ggtttgtgat tgggttgagg gtatggcagt gggaagtaat ttcatgcaag
1081 ttaattaaga gagcaaccac aaggcagcct taggtttatg aaagtcgtag aattatcaaa
1141 taccgcctgg agttagaagg aagcagtttc ttcctgtgca ttggatgcag acactttaaa
1201 tgttctctcc tctaccgtat gttcttggtt caaagtgtaa acttttctct gtgaagctgt
1261 taatcatcaa agatgtgagt tggtgaggtg gaggcgaatt ccttttgatt tcagaagaaa
1321 atatttgcga atctggccat ggaagccctc tctgaccttt tctccaaatt agaggaatta
1381 actgaacatg tgctaaggca catgaagctt ctttgtgcgg aattagagtg ctcatgttgg
1441 gagtgggcat cttgttaaac tcacaggata gatcaacttt ggatggattt tagacagtct
1501 atcacatgat agactttcag ataatcattt atggtactcc tgcatggtcc ggaatcccca
1561 ggtttcttgg agctttattg tcaaggaaaa gtacaagctt agccacagca atgtagagtt
1621 tctgctgctt agctctttgc aacacagtca tgttggatgc ttgagaaatt ggtgacctgc
1681 ccttccaagt cttaaaaatt gggggctggg taggacttgg aatgaggggg ttgtctgtta
1741 caaacttcat gtatcaagta ggttcagctt tgtccttatt tttcagtcaa aggaaagcca
1801 tagacaccaa tgtattccag gagggcttga ctccatccta gattcttccc atagcagttc
1861 ttgaatgcat ggctgcacgt gcacctctcc ccatttttgg ggtccttcat tggggtcagg
1921 atgaatgaag acatcaagct cactttgaag agcaagattc tgatttggac aggttcatcc
1981 ttctgctcat cacttgttcc ccttcaattg ataccctctc gttgttgggt tctgagaatt
2041 tgaaaccttg ctctgaattg aacatgaaaa agggccgtgt agccaacatc agggacagca
2101 gtgcttttct cagtgtgagt cttttaaaac ttgccttaca ggtaagcatc tgtctgtctt
2161 tatcgataga taaggaggct cttttggcta taaaccttta tagttttcct gttatcactc
2221 tgaggaatta ttgctttatg agagagtact ctctcaagag gaaaagaaag tcctagtttc
2281 tggtcatagc tacgaattaa atttatattc acgtctgttc agctctctga agttgttata
2341 ggtattttgg acaagaacta aagttagcta aagtttccac ataaccatca aagctgttat
2401 gaaaaaggga agaaggatgg accaggcagt aaggaggtca ttaaatcgtc cagagctttt
2461 cactaaaatt ggcagttcca gtgtccagcc taaatggctt gaatgtattt ttctctcttc
2521 tggtctcagc gaacatgctt gtcagctagt agttataaca gattgatggg tgaggcaaaa
2581 tacgcgaaag ttctttttgg ttacataaat actgcattag gactaagtgc agttgaagtt
2641 acaatattat taattaatcc ctccctttta aatttatatc tttgtgaagc ataggttagc
2701 aaatgccttt aaaaaatata tttcctttag gtcttaatga tctattcttt tggatagtct
2761 atttcacctg aatggattgt tgcttacaaa ttaaatatgt atcaggggct cccaatcctg
2821 aaagtcacct gaatttcctg gatctctgct agaaatcaac cttttaagag ataaaggagg
2881 gtttatccta aaagtactag ttacttctga aattcatgtc attactattg cttttggaga
2941 tataaataac aacaaaactt ttttttctta ctactataaa acatcatgga cttattggct
3001 ctatagatac agcccaggta agatttaaca ttttaaaaaa ttctattctg tcatctctaa
3061 tatgtttccc aattgtttca ggaccaggac gcccctacag ggccatagat ttctcacatc
3121 aaggacctgc atttgttacc tggcaccggt accatttgtt gtgtctggaa agagatctcc
3181 agcgactcat tggcaatgag tcttttgctt tgccctactg gaactttgcc actgggagga
3241 acgagtgtga tgtgtgtaca gaccagctgt ttggggcagc gagaccagac gatccgactc
3301 tgattagtcg gaactcaaga ttctccagct gggaaactgt ctgtgatagg taatggctcc
3361 tgtacctgca atggcatcca gagattgtct tttctgatgt ctgagacatg gaggagaagt
3421 cactttatag tgctgtttct tatgcatgtg gtgtcctatt tacatgtttc taaaaacagt
3481 tttcctctgg gaggtgggat ttcaaaaatt caaagctctt taatt
//