LOCUS CK335126 608 bp mRNA linear EST 12-MAY-2010 DEFINITION H3144F01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3144F01 5', mRNA sequence. ACCESSION CK335126 VERSION CK335126.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 608) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3144 row: F column: 01 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..608 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3144F01-5" /db_xref="taxon:10090" /clone="H3144F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 201 a 110 c 132 g 165 t ORIGIN 1 ggggtttata gcacaatagc taacaatcct aacaacagct ttgttgaaat tagaataaaa 61 gctcgtccaa gggaaatttg ctgaatgaag ttacccatca aacttaaagg ggctagaaag 121 ataacgttta catttaattg ctattcttca gttcttcatt aagtttccaa gttttagagt 181 ttaaggtatg gtgtgaagac tatatcctca gagtagtaga tttgccattc agtaagttct 241 gagacattat gaggtgccca cagtatgcaa aattggagag atactcaggt ggtcctcagt 301 agatcgatca tatgtggatc ttgtgcatgg aacctatcac cacccaaggt gagggcctaa 361 cacaactgag tcagcagttt acaaggaacc tagcaagaca gaacaattgt gcacctgtta 421 aaatgcctaa tcaatgctgt ataaaatatg acaggcactt aagcttaaaa ccaaaactgt 481 gcaagagctg cacttacatt tttgaagggg gttattagaa agtggcagag gtcattttgt 541 gctcacagat aaacactagg gaaaccagga atgttaccca gtattgtccc ctcaatggga 601 gacaagtg //