LOCUS CK334599 588 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061E12 5', mRNA sequence. ACCESSION CK334599 VERSION CK334599.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 588) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3061 row: E column: 12 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..588 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061E12-5" /db_xref="taxon:10090" /clone="H3061E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 169 c 151 g 122 t ORIGIN 1 gcgatcgcga gaaaaaaaat gcaacctccc aaaataaaga gcaaagattg cattaggagc 61 gaacagcgct gcagatatag atggcagctt cgtgtcattg actgaaacaa aacaaaacaa 121 aagggccact ggatgtctgc cttcttgggg ggtgagccag acagactgac aaacaaacag 181 acccagctga gctcggggga gggtttctcc tccggttttg cccggcagca gcagcatgga 241 cgtgttggct agttatagta tattccagga gctacaactt gtccacgaca ccggctactt 301 ctcagctttg ccatccctgg aggagacctg gcagcagaca tgccttgagt tggaacgcta 361 cctccagaca gaaccccggc ggatctcgga gacctttggt gaggacttgg actgcttcct 421 ccacgcttct cctcccccat gcattgagga gagcttccgg cgcctagacc ccttgctgct 481 cccagtggaa gccaccatct gcgagaagag ctccgcagtg gacattttgc tctctcggga 541 caagttgcta tctgagacct gcctcagcct ccagccaacc agctcttc //