LOCUS CK334599 588 bp mRNA linear EST 12-MAY-2010
DEFINITION H3061E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3061E12 5', mRNA sequence.
ACCESSION CK334599
VERSION CK334599.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 588)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3061 row: E column: 12
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..588
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3061E12-5"
/db_xref="taxon:10090"
/clone="H3061E12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 146 a 169 c 151 g 122 t
ORIGIN
1 gcgatcgcga gaaaaaaaat gcaacctccc aaaataaaga gcaaagattg cattaggagc
61 gaacagcgct gcagatatag atggcagctt cgtgtcattg actgaaacaa aacaaaacaa
121 aagggccact ggatgtctgc cttcttgggg ggtgagccag acagactgac aaacaaacag
181 acccagctga gctcggggga gggtttctcc tccggttttg cccggcagca gcagcatgga
241 cgtgttggct agttatagta tattccagga gctacaactt gtccacgaca ccggctactt
301 ctcagctttg ccatccctgg aggagacctg gcagcagaca tgccttgagt tggaacgcta
361 cctccagaca gaaccccggc ggatctcgga gacctttggt gaggacttgg actgcttcct
421 ccacgcttct cctcccccat gcattgagga gagcttccgg cgcctagacc ccttgctgct
481 cccagtggaa gccaccatct gcgagaagag ctccgcagtg gacattttgc tctctcggga
541 caagttgcta tctgagacct gcctcagcct ccagccaacc agctcttc
//