LOCUS BG077650 662 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018B01 5', mRNA sequence. ACCESSION BG077650 VERSION BG077650.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 662) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077650.1. Other_ESTs: H3018B01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: B column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..662 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018B01-5" /db_xref="taxon:10090" /clone="H3018B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 215 c 180 g 135 t ORIGIN 1 cctcaccctc cgcggacctc ttagggctgc gggcagcccc tccccctgct gcacccccgg 61 ctcccgtagg cgggaacctc ctggtggatg tcttctctga cggccccact gcacagccca 121 gcctggggcc cactcctgag gaggccttcc tcagcccagg tcctgaggac ataggccctc 181 ccattccaga agcagatgaa ctgttgaata agttcgtgtg taagaatagt ggggtcttgt 241 ttgagaacca gctgctgcag attggagtca agtctgagtt ccggcagaac ctgggccgca 301 tgtatctctt ctatggcaac aagacttctg tgcagttcca gaacttcttg cccaccgtgg 361 tccatcctgg ggacctccag actcagctgg cggtgcagac caagcgtgtg gcggcacaag 421 tgggcggtgg cgcacaggtg cagcaagtac tcaacattga gtgtctgcga gacttcctga 481 cgccgccact gttgtcggtg cgcttccggt acggtggcac cgcccagtcc ctcactctga 541 agctcccagt gaccatcaac aaattcttcc agcccacaga gatggcggcc caagactttt 601 tccagcgctg gaagcagctg agcctccccc tgcaggaggc acagaaaatc ttcaaagcca 661 ac //