LOCUS       BG077650                 662 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3018B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3018B01 5', mRNA sequence.
ACCESSION   BG077650
VERSION     BG077650.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077650.1.
            Other_ESTs: H3018B01-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3018  row: B  column: 01
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3018B01-5"
                     /db_xref="taxon:10090"
                     /clone="H3018B01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          132 a          215 c          180 g          135 t
ORIGIN      
        1 cctcaccctc cgcggacctc ttagggctgc gggcagcccc tccccctgct gcacccccgg
       61 ctcccgtagg cgggaacctc ctggtggatg tcttctctga cggccccact gcacagccca
      121 gcctggggcc cactcctgag gaggccttcc tcagcccagg tcctgaggac ataggccctc
      181 ccattccaga agcagatgaa ctgttgaata agttcgtgtg taagaatagt ggggtcttgt
      241 ttgagaacca gctgctgcag attggagtca agtctgagtt ccggcagaac ctgggccgca
      301 tgtatctctt ctatggcaac aagacttctg tgcagttcca gaacttcttg cccaccgtgg
      361 tccatcctgg ggacctccag actcagctgg cggtgcagac caagcgtgtg gcggcacaag
      421 tgggcggtgg cgcacaggtg cagcaagtac tcaacattga gtgtctgcga gacttcctga
      481 cgccgccact gttgtcggtg cgcttccggt acggtggcac cgcccagtcc ctcactctga
      541 agctcccagt gaccatcaac aaattcttcc agcccacaga gatggcggcc caagactttt
      601 tccagcgctg gaagcagctg agcctccccc tgcaggaggc acagaaaatc ttcaaagcca
      661 ac
//