LOCUS BG072203 601 bp mRNA linear EST 12-MAY-2010 DEFINITION H3107H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3107H10 3', mRNA sequence. ACCESSION BG072203 VERSION BG072203.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 601) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072203.1. Other_ESTs: H3107H10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3107 row: H column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..601 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3107H10-3" /db_xref="taxon:10090" /clone="H3107H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 149 c 129 g 176 t ORIGIN 1 cagatgttca gagtcttctt tactgcaaat atgatgtctt tgacctgagg tacggagttg 61 tcttctagga cctttgcgta aggcataggg acatcagcac cagtgacacg cacggcaggg 121 gcatcaagga agttgaatgc agggccttcc ataattctgg cacaaatctc agctcctact 181 ccaaattgtg gccagcctcc ttccacagtc acgagatgat ttgtcttcat cacactggct 241 tctatggctt cgatgtccat tggtctgatg gtgcgcagat ttattacctc acattcaatt 301 ccctccttag acaatacagc tgcagcttct aggcagtggc ccactggtct tgaatgggca 361 actacagtga tgtgggtccc ttgcctttca attttggctt ttccaatagg aatcaggaaa 421 tcttttgact gagcttctgc aggaagttca aatgcaactc catacatcag ttcattttct 481 agcattacca ctgggttatt atcacgaatg gccgatttaa taagtccttt tgcatcctcg 541 gaattccagg gactgaccac ttttaaacct gggcagtgcc cataccatgc agcaaagcat 601 t //