LOCUS BG070265 545 bp mRNA linear EST 12-MAY-2010 DEFINITION H3085E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3085E01 3', mRNA sequence. ACCESSION BG070265 VERSION BG070265.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 545) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070265.1. Other_ESTs: H3085E01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3085 row: E column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..545 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3085E01-3" /db_xref="taxon:10090" /clone="H3085E01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 195 a 100 c 93 g 157 t ORIGIN 1 tgacgaaagt aaagtattgc tccagtcaca gcaccaggtc acactcgtca acgtccacat 61 cctccgagtg ccctggacac acaggatggg ccagccgaca ccgccactgc tcaccatgga 121 tttttggttt gttcatatga aaatattgcg gcaaatgaaa gaaacaaaga attcatcacc 181 gtttgctata agaattgtgc ttattaacac gtgataataa aggcattgtt tttcttcata 241 cgattgcaaa gcagattttt tatgctttca aactcaattc ttttaataaa cagtaacaaa 301 ttctctgtta agatgttcaa atggagacaa aaaaataagt ccagctgttg gaagaaattc 361 aataaatagc tattttacat ggataaagtc atagtggtac aatttatgaa tgtcacatca 421 ggcatgcaca aaaatgtatc atacatgaca ggagcagtca gaaaatattg aattaggtct 481 aaaaagatat gaagaattta ctgtacacat atatacaaaa atcttgcaaa ttattgcctc 541 atttt //