LOCUS       BG070265                 545 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3085E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3085E01 3', mRNA sequence.
ACCESSION   BG070265
VERSION     BG070265.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG070265.1.
            Other_ESTs: H3085E01-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3085  row: E  column: 01
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3085E01-3"
                     /db_xref="taxon:10090"
                     /clone="H3085E01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          195 a          100 c           93 g          157 t
ORIGIN      
        1 tgacgaaagt aaagtattgc tccagtcaca gcaccaggtc acactcgtca acgtccacat
       61 cctccgagtg ccctggacac acaggatggg ccagccgaca ccgccactgc tcaccatgga
      121 tttttggttt gttcatatga aaatattgcg gcaaatgaaa gaaacaaaga attcatcacc
      181 gtttgctata agaattgtgc ttattaacac gtgataataa aggcattgtt tttcttcata
      241 cgattgcaaa gcagattttt tatgctttca aactcaattc ttttaataaa cagtaacaaa
      301 ttctctgtta agatgttcaa atggagacaa aaaaataagt ccagctgttg gaagaaattc
      361 aataaatagc tattttacat ggataaagtc atagtggtac aatttatgaa tgtcacatca
      421 ggcatgcaca aaaatgtatc atacatgaca ggagcagtca gaaaatattg aattaggtct
      481 aaaaagatat gaagaattta ctgtacacat atatacaaaa atcttgcaaa ttattgcctc
      541 atttt
//