LOCUS BC014678 2271 bp mRNA linear HUM 12-FEB-2004 DEFINITION Homo sapiens leucine rich repeat and fibronectin type III domain containing 1, mRNA (cDNA clone IMAGE:3860672), partial cds. ACCESSION BC014678 VERSION BC014678.1 KEYWORDS . SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 2271) AUTHORS Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G., Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D., Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K., Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F., Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L., Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L., Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S., Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J., Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J., McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S., Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W., Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A., Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S., Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y., Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D., Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M., Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E., Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A. TITLE Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences JOURNAL Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) PUBMED 12477932 REFERENCE 2 (bases 1 to 2271) AUTHORS Strausberg,R. TITLE Direct Submission JOURNAL Submitted (01-OCT-2001) National Institutes of Health, Mammalian Gene Collection (MGC), Cancer Genomics Office, National Cancer Institute, 31 Center Drive, Room 11A03, Bethesda, MD 20892-2590, USA REMARK NIH-MGC Project URL: http://mgc.nci.nih.gov COMMENT Contact: MGC help desk Email: cgapbs-r@mail.nih.gov Tissue Procurement: DCTD/DTP cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Baylor College of Medicine Human Genome Sequencing Center Center code: BCM-HGSC Web site: http://www.hgsc.bcm.tmc.edu/cdna/ Contact: amg@bcm.tmc.edu Gunaratne, P.H., Garcia, A.M., Lu, X., Hulyk, S.W., Loulseged, H., Kowis, C.R., Sneed, A.J., Martin, R.G., Muzny, D.M., Nanavati, A.N., Gibbs, R.A. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Series: IRAK Plate: 21 Row: e Column: 22 This clone was selected for full length sequencing because it passed the following selection criteria: Hexamer frequency ORF analysis, Similarity but not identity to protein. FEATURES Location/Qualifiers source 1..2271 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="IMAGE:3860672" /tissue_type="Ovary, adenocarcinoma" /clone_lib="NIH_MGC_66" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene <1..2271 /gene="LRFN1" /gene_synonym="KIAA1484" /db_xref="GeneID:57622" CDS <1..1355 /gene="LRFN1" /gene_synonym="KIAA1484" /codon_start=3 /product="LRFN1 protein" /protein_id="AAH14678.1" /db_xref="GeneID:57622" /translation="RQAGGRALVVEGQAVSLRCRAVGDPEPVVHWVAPDGRLLGNSSR TRVRGDGTLDVTITTLRDSGTFTCIASNAAGEATAPVEVCVVPLPLMAPPPAAPPPLT EPGSSDIATPGRPGANDSAAERRLVAAELTSNSVLIRWPAQRPVPGIRMYQVQYNSSV DDSLVYRMIPSTSQTFLVNDLAAGRAYDLCVLAVYDDGATALPATRVVGCVQFTTAGD PAPCRPLRAHFLGGTMIIAIGGVIVASVLVFIVLLMIRYKVYGDGDSRRVKGSRSLPR VSHVCSQTNGAGTGAAQAPALPAQDHYEALREVESQAAPAVAVEAKAMEAETASAEPE VVLGRSLGGSATSLCLLPSEETSGEESRAAVGPRRSRSGALEPPTSAPPTLALVPGGA AARPRPQQRYSFDGDYGALFQSHSYPRRARRTKRHRSTLHSSLGNRARLCLRKKKRNI " misc_feature 18..251 /gene="LRFN1" /gene_synonym="KIAA1484" /note="IGcam; Region: Immunoglobulin domain cell adhesion molecule (cam) subfamily" /db_xref="CDD:cd00931" misc_feature 372..596 /gene="LRFN1" /gene_synonym="KIAA1484" /note="fn3; Region: Fibronectin type III domain" /db_xref="CDD:pfam00041" BASE COUNT 558 a 602 c 604 g 507 t ORIGIN 1 cacggcaggc ggggggccgg gccctggtgg tggaaggcca ggcggtgagc ctgcgctgcc 61 gagcggtggg tgaccccgag ccggtggtgc actgggtggc acctgatggg cggctgctgg 121 ggaactccag ccggacccgg gtccgggggg acgggacgct ggatgtgacc atcaccacct 181 tgagggacag tggcaccttc acttgtatcg cctccaatgc tgctggggaa gcgacggcgc 241 ccgtggaggt gtgcgtggta cctctgcctc tgatggcacc cccgccggct gccccgccgc 301 ctctcaccga gcccggctcc tctgacatcg ccacgccggg cagaccaggt gccaacgatt 361 ctgcggctga gcgtcggctc gtggcagccg agctcacctc gaactccgtg ctcatccgct 421 ggccagccca gaggcctgtg cccggaatac gcatgtacca ggttcagtac aacagttccg 481 ttgatgactc cctcgtctac aggatgatcc cgtccaccag tcagaccttc ctggtgaatg 541 acctggcggc gggccgtgcc tacgacttgt gcgtgctggc ggtctacgac gacggggcca 601 cagcgctgcc ggcaacgcga gtggtgggct gtgtacagtt caccaccgct ggggatccgg 661 cgccctgccg cccgctgagg gcccatttct tgggcggcac catgatcatc gccatcgggg 721 gcgtcatcgt cgcctcggtc ctcgtcttca tcgttctgct catgatccgc tataaggtgt 781 atggcgacgg ggacagccgc cgcgtcaagg gctccaggtc gctcccgcgg gtcagccacg 841 tgtgctcgca gaccaacggc gcaggcacag gcgcggcaca ggccccggcc ctgccggccc 901 aggaccacta cgaggcgctg cgcgaggtgg agtcccaggc tgcccccgcc gtcgccgtcg 961 aggccaaggc catggaggcc gagacggcat ccgcggagcc ggaggtggtc cttggacgtt 1021 ctctgggcgg ctcggccacc tcgctgtgcc tgctgccatc cgaggaaact tccggggagg 1081 agtctcgggc cgcggtgggc cctcgaagga gccgatccgg cgccctggag ccaccaacct 1141 cggcgccccc tactctagct ctagttcctg ggggagccgc ggcccggccg aggccgcagc 1201 agcgctattc gttcgacggg gactacgggg cactattcca gagccacagt tacccgcgcc 1261 gcgcccggcg gacaaagcgc caccggtcca cgctgcactc cagcctgggc aacagagcga 1321 gactctgtct cagaaaaaaa aaaagaaata tctgacagta gaggacagat taaataaatt 1381 atgatctgct atatcatgac ataaaataca tttactagtg atactgatcc atataattga 1441 gaaagcattt atgatgtatt accaaatgaa gaaagtagat ttaaaaaggt acatgtaatt 1501 tgggcagagg tactttggag aaattaagaa cacaggctct gcagtacaac tttcagggtg 1561 tgattctgaa ctctgacagt tactaacctc gcagtgtctt tttcctcact ggtgaaactg 1621 gaatataaat accatgagat atggctatct caattatcaa gtaagataat ctacataaaa 1681 catagtaata cataacaatt actttacata agttagctgt ttttattaaa gatcactgac 1741 attacatatc ccttaatcca tttttagaaa agaaacatga ggctgctgtt ttcttatgct 1801 aaaatatgtg cttctcaatc agctatcaat atcatgaatg ctaactaaaa ataactttcc 1861 aagcctgatg ttaagcatga atttatttga atcagcagaa ttagcaaaaa cgattctaag 1921 acacgtaaga aaatgttccc tctttaccag caactaggga aatagagaca tgattctgca 1981 tcagaaatca agtttaaaga atctaatctg agaagagata ccaaatcaag aatattagca 2041 agagactgaa tcatgtgttc tatgtgatat gggagattcg tgtaattatt tcaaattgtt 2101 gccatcttag tcatttcata tatttggatt tcatatacct tggatttgtt acttgtacat 2161 ataatcatgg aaatgttact tgtgtatatt tggaaggcat cagatatcac tgattatcat 2221 tctctgtcaa agaaacatta aaatcataga aaatctaaaa aaaaaaaaaa a //