LOCUS LT727410 5815 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSp35, complete sequence.
ACCESSION LT727410
VERSION LT727410.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSp35
ORGANISM Mammalian expression vector pCAGGSp35
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5815)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5815
/organism="Mammalian expression vector pCAGGSp35"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3829."
/db_xref="taxon:1944891"
misc_feature 4..21
/note="5UTR"
CDS 22..924
/transl_table=11
/note="p35"
/protein_id="SJL88369.1"
/translation="MMCVIFPVEIDVSQTIIRDCQVDKQTRELVYINKIMNTQLTKPV
LMMFNISGPIRSVTRKNNNLRDRIKSKVDEQFDQLERDYSDQMDGFHDSIKYFKDEHY
SVSCQNGSVLKSKFAKILKSHDYTDKKSIEAYEKYCLPKLVDERNDYYVAVCVLKPGF
ENGSNQVLSFEYNPIGNKVIVPFAHEINDTGLYEYDVVAYVDSVQFDGEQFEEFVQSL
ILPSSFKNSEKVLYYNEASKNKSMIYKALEFTTESSWGKSEKYNWKIFCNGFIYDKKS
KVLYVKLHNVTSALNKNVILNTIK"
misc_feature 925..1023
/note="3UTR"
regulatory 1105..1196
/note="polyA"
/regulatory_class="terminator"
misc_feature 1197..1553
/note="3FR"
regulatory complement(1631..1713)
/note="lac"
/regulatory_class="promoter"
rep_origin 1855..1930
/note="SV40 ori"
regulatory 1961..2035
/note="polyA"
/regulatory_class="terminator"
regulatory complement(1961..2035)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(2150..2551)
/note="pMB1 ori"
CDS complement(3092..3952)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88370.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 4088..4465
/note="hCMV"
regulatory 4471..5812
/note="AG"
/regulatory_class="promoter"
misc_feature 4840..5764
/note="intron"
BASE COUNT 1350 a 1434 c 1546 g 1485 t
ORIGIN
1 ttcgatcttt accatagcaa aatgatgtgt gtaatttttc cggtagaaat cgacgtgtcc
61 cagacgatta ttcgagattg tcaggtggac aaacaaacca gagagttggt gtacattaac
121 aagattatga acacgcaatt gacaaaaccc gttctcatga tgtttaacat ttcgggtcct
181 atacgaagcg ttacgcgcaa gaacaacaat ttgcgcgaca gaataaaatc aaaagtcgat
241 gaacaatttg atcaactaga acgcgattac agcgatcaaa tggatggatt ccacgatagc
301 atcaagtatt ttaaagatga acactattcg gtaagttgcc aaaatggcag cgtgttgaaa
361 agcaagtttg ctaaaatttt aaagagtcat gattataccg ataaaaagtc tattgaagct
421 tacgagaaat actgtttgcc caaattggtc gacgaacgca acgactacta cgtggcggta
481 tgcgtgttga agccgggatt tgagaacggc agcaaccaag tgctatcttt cgagtacaac
541 ccgattggta acaaagttat tgtgccgttt gctcacgaaa ttaacgacac gggactttac
601 gagtacgacg tcgtagctta cgtggacagt gtgcagtttg atggcgaaca atttgaagag
661 tttgtgcaga gtttaatatt gccgtcgtcg ttcaaaaatt cggaaaaggt tttatattac
721 aacgaagcgt cgaaaaacaa aagcatgatc tacaaggctt tagagtttac tacagaatcg
781 agctggggca aatccgaaaa gtataattgg aaaatttttt gtaacggttt tatttatgat
841 aaaaaatcaa aagtgttgta tgttaaattg cacaatgtaa ctagtgcact caacaaaaat
901 gtaatattaa acacaattaa ataaatgtta aaatttattg cctaatatta ttttgtcatt
961 gcttgtcatt tattaatttg gatgatgtca tttgttttta aaattgaact ggctttacga
1021 gtagaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
1081 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
1141 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
1201 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
1261 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
1321 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
1381 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
1441 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
1501 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
1561 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
1621 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa
1681 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
1741 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
1921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
1981 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
2041 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
2101 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
2221 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag
2281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
2521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
2941 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
3001 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3061 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3121 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3181 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3241 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3301 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3361 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
3421 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3481 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
3541 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
3601 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
3661 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
3721 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
3781 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
3841 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
3901 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
3961 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4021 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
4081 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
4441 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
4501 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
4561 gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag
4621 gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga
4681 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg
4741 cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc
4801 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
4861 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
4921 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
4981 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
5041 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
5101 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
5161 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
5221 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
5281 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
5341 ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag
5401 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
5461 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
5521 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
5581 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
5641 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
5701 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
5761 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa
//