LOCUS LT727384 7278 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGSEhA20R562A, complete sequence.
ACCESSION LT727384
VERSION LT727384.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGSEhA20R562A
ORGANISM Mammalian expression vector pCAGGSEhA20R562A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7278)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7278
/organism="Mammalian expression vector pCAGGSEhA20R562A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3777."
/db_xref="taxon:1944767"
misc_feature 16..54
/note="E-tag"
CDS 64..2436
/transl_table=11
/note="hA20m"
/protein_id="SJL88309.1"
/translation="MAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREVRKLVALKTNGDGN
CLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLIKMASTDTPMARSGLQYNSLEEIHIFVLCNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCETPNC
PFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASRGEAYEPLAWNP
EESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHNARQLHASH
APDHTRHLDPGKCQACLQDVTRTFNGICSTCFKRTTAEASSSLSTSLPPSCHQASKSD
PSRLVRSPSPHSCHRAGNDAPAGCLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFC
TLCFIEYRENKHFAAASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQ
NQRFHEAKRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHPN
QRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMYG"
regulatory 2568..2659
/note="polyA"
/regulatory_class="terminator"
misc_feature 2660..3016
/note="3FR"
regulatory complement(3094..3176)
/note="lac"
/regulatory_class="promoter"
rep_origin 3318..3393
/note="SV40 ori"
regulatory 3424..3498
/note="polyA"
/regulatory_class="terminator"
regulatory complement(3424..3498)
/note="polyA"
/regulatory_class="terminator"
rep_origin complement(3613..4014)
/note="pMB1 ori"
CDS complement(4555..5415)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88310.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5551..5928
/note="hCMV"
/regulatory_class="promoter"
regulatory 5934..7275
/note="AG"
/regulatory_class="promoter"
misc_feature 6303..7227
/note="intron"
BASE COUNT 1660 a 1953 c 1968 g 1697 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaatggctg aacaagtcct tcctcaggct ttgtatttga gcaatatgcg gaaagctgtg
121 aagatacggg agagaactcc agaagacatt tttaaaccta ctaatgggat cattcatcat
181 tttaaaacca tgcaccgata cacactggaa atgttcagaa cttgccagtt ttgtcctcag
241 tttcgggaga tcatccacaa agccctcatc gacagaaaca tccaggccac cctggaaagc
301 cagaagaaac tcaactggtg tcgagaagtc cggaagcttg tggcgctgaa aacgaacggt
361 gacggcaatt gcctcatgca tgccacttct cagtacatgt ggggcgttca ggacacagac
421 ttggtactga ggaaggcgct gttcagcacg ctcaaggaaa cagacacacg caactttaaa
481 ttccgctggc aactggagtc tctcaaatct caggaatttg ttgaaacggg gctttgctat
541 gatactcgga actggaatga tgaatgggac aatcttatca aaatggcttc cacagacaca
601 cccatggccc gaagtggact tcagtacaac tcactggaag aaatacacat atttgtcctt
661 tgcaacatcc tcagaaggcc aatcattgtc atttcagaca aaatgctaag aagtttggaa
721 tcaggttcca atttcgcccc tttgaaagtg ggtggaattt acttgcctct ccactggcct
781 gcccaggaat gctacagata ccccattgtt ctcggctatg acagccatca ttttgtaccc
841 ttggtgaccc tgaaggacag tgggcctgaa atccgagctg ttccacttgt taacagagac
901 cggggaagat ttgaagactt aaaagttcac tttttgacag atcctgaaaa tgagatgaag
961 gagaagctct taaaagagta cttaatggtg atagaaatcc ccgtccaagg ctgggaccat
1021 ggcacaactc atctcatcaa tgccgcaaag ttggatgaag ctaacttacc aaaagaaatc
1081 aatctggtag atgattactt tgaacttgtt cagcatgagt acaagaaatg gcaggaaaac
1141 agcgagcagg ggaggagaga ggggcacgcc cagaatccca tggaaccttc cgtgccccag
1201 ctttctctca tggatgtaaa atgtgaaacg cccaactgcc ccttcttcat gtctgtgaac
1261 acccagcctt tatgccatga gtgctcagag aggcggcaaa agaatcaaaa caaactccca
1321 aagctgaact ccaagccggg ccctgagggg ctccctggca tggcgctcgg ggcctctcgg
1381 ggagaagcct atgagccctt ggcgtggaac cctgaggagt ccactggggg gcctcattcg
1441 gccccaccga cagcacccag cccttttctg ttcagtgaga ccactgccat gaagtgcagg
1501 agccccggct gccccttcac actgaatgtg cagcacaacg gattttgtga acgttgccac
1561 aacgcccggc aacttcacgc cagccacgcc ccagaccaca caaggcactt ggatcccggg
1621 aagtgccaag cctgcctcca ggatgttacc aggacattta atgggatctg cagtacttgc
1681 ttcaaaagga ctacagcaga ggcctcctcc agcctcagca ccagcctccc tccttcctgt
1741 caccaggcta gcaagtcaga tccctcgcgg ctcgtccgga gcccctcccc gcattcttgc
1801 cacagagctg gaaacgacgc ccctgctggc tgcctgtctc aagctgcacg gactcctggg
1861 gacaggacgg ggacgagcaa gtgcagaaaa gccggctgcg tgtattttgg gactccagaa
1921 aacaagggct tttgcacact gtgtttcatc gagtacagag aaaacaaaca ttttgctgct
1981 gcctcaggga aagtcagtcc cacagcgtcc aggttccaga acaccattcc gtgcctgggg
2041 agggaatgcg gcacccttgg aagcaccatg tttgaaggat actgccagaa gtgtttcatt
2101 gaagctcaga atcagagatt tcatgaggcc aaaaggacag aagagcaact gagatcgagc
2161 cagcgcagag atgtgcctcg aaccacacaa agcacctcaa ggcccaagtg cgcccgggcc
2221 tcctgcaaga acatcctggc ctgccgcagc gaggagctct gcatggagtg tcagcatccc
2281 aaccagagga tgggccctgg ggcccaccgg ggtgagcctg cccccgaaga cccccccaag
2341 cagcgttgcc gggcccccgc ctgtgatcat tttggcaatg ccaagtgcaa cggctactgc
2401 aacgaatgct ttcagttcaa gcagatgtat ggctaaccgg aaacaggtgg gtcacctcct
2461 gcaagaagtg gggcctcgag gtcgaggaat tcactcctca ggtgcaggct gcctatcaga
2521 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
2581 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
2641 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
2701 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
2761 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
2821 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
2881 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
2941 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
3001 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
3061 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
3121 aagtgtaaag cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
3181 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc
3241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
3421 gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
3541 tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
3601 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga
3721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
3781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
4021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
4081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
4141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
4201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
4261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
4321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
4381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
5041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
5101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
5161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
5221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
5281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
5341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
5401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5521 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt
5581 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
5641 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
5701 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
5761 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
5821 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
5881 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
5941 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
6001 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc
6061 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag
6121 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
6181 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc
6241 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
6301 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
6361 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
6421 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
6481 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
6541 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
6601 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
6661 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
6721 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
6781 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
6841 aaggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
6901 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
6961 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
7021 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
7081 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
7141 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
7201 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
7261 catcattttg gcaaagaa
//