LOCUS LT727313 6694 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-FLAGmA20f2, complete sequence.
ACCESSION LT727313
VERSION LT727313.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-FLAGmA20f2
ORGANISM Mammalian expression vector pCAGGS-FLAGmA20f2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6694)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6694
/organism="Mammalian expression vector pCAGGS-FLAGmA20f2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4122."
/db_xref="taxon:1944855"
misc_feature 13..36
/note="FLAG epitope"
CDS 46..>1965
/transl_table=11
/note="mA20 (1-640)"
/protein_id="SJL88080.1"
/translation="MAEQLLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIYHFKTMH
RYTLEMFRTCQFCPQFREIIHKALIDRSVQASLESQKKLNWCREVRKLVALKTNGDGN
CLMHAACQYMWGVQDTDLVLRKALCSTLKETDTRNFKFRWQLESLKSQEFVETGLCYD
TRNWNDEWDNLVKMASADTPAARSGLQYNSLEEIHIFVLSNILRRPIIVISDKMLRSL
ESGSNFAPLKVGGIYLPLHWPAQECYRYPIVLGYDSQHFVPLVTLKDSGPELRAVPLV
NRDRGRFEDLKVHFLTDPENEMKEKLLKEYLIVMEIPVQGWDHGTTHLINAAKLDEAN
LPKEINLVDDYFELVQHEYKKWQENSDQARRAAHAQNPLEPSTPQLSLMDIKCETPNC
PFFMSVNTQPLCHECSERRQKNQSKLPKLNSKLGPEGLPGVGLGSSNWSPEETAGGPH
SAPPTAPSLFLFSETTAMKCRSPGCPFTLNVQHNGFCERCHARQINASHTADPGKCQA
CLQDVTRTFNGICSTCFKRTTAEPSSSLTSSIPASCHQRSKSDPSQLIQSLTPHSCHR
TGNVSPSGCLSQAARTPGDRAGTSKCRKAGCMYFGTPENKGFCTLCFIEYRENKQSVT
ASAKAGSPAPRFQNNV"
regulatory 1980..2071
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 2072..2428
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2506..2588)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2730..2805
/note="SV40 ori"
regulatory 2836..2910
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2836..2910)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3025..3426)
/note="pMB1 ori"
CDS complement(3967..4827)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88081.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4967..5344
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5350..6691
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5719..6643
/note="intron AG promoter"
BASE COUNT 1531 a 1774 c 1778 g 1611 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt
61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc
121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga
181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac
241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg
301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg
361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc
421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa
481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat
541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga
601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga
661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct
721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga
781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac
841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac
901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag
961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc
1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac
1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga
1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata
1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac
1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta
1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc
1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc
1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga
1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag
1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc
1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc ctcctgtcac
1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac
1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac
1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac
1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgcc
1921 tctgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtctaagt gtcgacgaag
1981 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
2041 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
2101 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt
2161 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
2221 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
2281 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
2341 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
2401 tagctgtccc tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca
2461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
2521 gccggaagca taaagtgtaa agcctgggtg cctaatgagt gagctaactc acattaattg
2581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
2821 cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc
2881 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
2941 ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg
3001 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
3061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
3121 cagaatcagg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
3181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
3661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
3841 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
3901 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
3961 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
4021 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
4081 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
4141 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
4201 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
4261 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
4321 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
4381 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
4441 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
4501 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
4561 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
4621 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
4681 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
4741 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
4801 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
4861 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
4921 aggggttccg cgcacatttc cccgaaaagt gccacctggg tcgatcgaca ttgattattg
4981 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
5041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
5101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
5161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
5221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
5281 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
5341 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
5401 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
5461 gggggggggc cccccccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
5521 aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
5581 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgttgcgcgc
5641 tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
5701 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
5761 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
5821 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
5881 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
5941 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
6001 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
6061 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
6121 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
6181 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
6241 ggggagggct cgggggaagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
6301 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
6361 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
6421 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
6481 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
6541 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
6601 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
6661 ggttattgtg ctgtctcatc attttggcaa agaa
//