LOCUS LT726918 7082 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A, complete
sequence.
ACCESSION LT726918
VERSION LT726918.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A
ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-W740A
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7082)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7082
/organism="Mammalian expression vector
pCAGGS-Flag-IKKalpha-W740A"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5491."
/db_xref="taxon:1944851"
misc_feature 13..36
/note="FLAG epitope"
CDS 43..2280
/transl_table=11
/note="hIKKalpha-W740A"
/protein_id="SJL86937.1"
/translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK
SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD
LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII
HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY
RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL
QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL
HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT
VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA
AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKML
KAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAID
LYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPK
VEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAW
LPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDA
SWLTE"
misc_feature 2260..2265
/note="W740A"
regulatory 2368..2459
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 2460..2816
/note="3' FR of beta-globin"
regulatory complement(2894..2976)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 3118..3193
/note="SV40 ori"
regulatory 3224..3298
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3224..3298)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(3413..3814)
/note="pMB1 ori"
CDS complement(4355..5215)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86938.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 5355..5732
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 5738..7079
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 6107..7031
/note="intron AG promoter"
BASE COUNT 1705 a 1693 c 1885 g 1799 t
ORIGIN
1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg
61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc
121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt
181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag
241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg
301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg
361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt
421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa
481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg
541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag
601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc
661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag
721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa
781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt
841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag
901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac
961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt
1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga
1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa
1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg
1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt
1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg
1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag
1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag
1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaaagc
1501 attcagcttg acttggagag atacagcgag cagatgacgt atgggatatc ttcagaaaaa
1561 atgctaaaag catggaaaga aatggaagaa aaggccatcc actatgctga ggttggtgtc
1621 attggatacc tggaggatca gattatgtct ttgcatgctg aaatcatgga gctacagaag
1681 agcccctatg gaagacgtca gggagacttg atggaatctc tggaacagcg tgccattgat
1741 ctatataagc agttaaaaca cagaccttca gatcactcct acagtgacag cacagagatg
1801 gtgaaaatca ttgtgcacac tgtgcagagt caggaccgtg tgctcaagga gctgtttggt
1861 catttgagca agttgttggg ctgtaagcag aagattattg atctactccc taaggtggaa
1921 gtggccctca gtaatatcaa agaagctgac aatactgtca tgttcatgca gggaaaaagg
1981 cagaaagaaa tatggcatct ccttaaaatt gcctgtacac agagttctgc ccggtccctt
2041 gtaggatcca gtctagaagg tgcagtaacc cctcagacat cagcatggct gcccccgact
2101 tcagcagaac atgatcattc tctgtcatgt gtggtaactc ctcaagatgg ggagacttca
2161 gcacaaatga tagaagaaaa tttgaactgc cttggccatt taagcactat tattcatgag
2221 gcaaatgagg aacagggcaa tagtatgatg aatcttgatg ccagctggtt aacagaatga
2281 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
2341 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
2401 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
2461 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
2521 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
2581 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
2641 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
2701 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
2761 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
2821 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
2881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc
2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
3001 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
3061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
3121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
3181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
3361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
3421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
3481 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt
3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
5341 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
5401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
5461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
5521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
5581 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
5641 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
5701 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
5761 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
5821 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg
5881 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc
5941 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
6001 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc
6061 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
6121 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
6181 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
6241 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
6301 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
6361 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
6421 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
6481 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
6541 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
6601 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc
6661 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
6721 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
6781 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
6841 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
6901 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
6961 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
7021 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
7081 aa
//