LOCUS       LC610786                 617 bp    DNA     linear   BCT 26-FEB-2021
DEFINITION  Escherichia sp. HW_Hon9 tetM gene for tetracycline resistance
            protein, partial cds.
ACCESSION   LC610786
VERSION     LC610786.1
KEYWORDS    .
SOURCE      Escherichia sp.
  ORGANISM  Escherichia sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Matsui,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2021)
            Contact:Kazuaki Matsui
            Kindai University, Civil and Environmental Engineering; 3-4-1
            Kowakae, Higashiosaka, Osaka 5778502, Japan
            URL    :https://www.kindai.ac.jp/science-engineering/english/
            education/teachers/detail/08_matsui_kazuaki.html
REFERENCE   2
  AUTHORS   Matsui,K.
  TITLE     Quantification of tetracycline resistance genes in Japanese urban
            waterway
  JOURNAL   Unpublished
COMMENT     
FEATURES             Location/Qualifiers
     source          1..617
                     /collected_by="Kazuaki Matsui"
                     /collection_date="2017-09-07"
                     /db_xref="taxon:1884818"
                     /geo_loc_name="Japan: Osaka, Higashiyokoborigawa waterway"
                     /isolate="HW_Hon9"
                     /isolation_source="waterway water"
                     /lat_lon="34.68 N 135.50 E"
                     /mol_type="genomic DNA"
                     /organism="Escherichia sp."
                     /PCR_primers="fwd_seq: gttaaatagtgttcttggag, rev_seq:
                     ctaagatatggctctaacaa"
     CDS             <1..>617
                     /codon_start=3
                     /gene="tetM"
                     /product="tetracycline resistance protein"
                     /protein_id="BCT04098.1"
                     /transl_table=11
                     /translation="TKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDP
                     LLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYT
                     IHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGL
                     YGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTE"
BASE COUNT          200 a          110 c          130 g          177 t
ORIGIN      
        1 atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta ctacaaacaa
       61 ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt ttggaaatct
      121 cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa attatacttt
      181 ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg
      241 tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag
      301 aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg
      361 tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact
      421 taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat
      481 tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc
      541 ctgttagtac cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa
      601 aaaaagctgg aacagaa
//