LOCUS JO315240 623 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c51737_c mRNA sequence. ACCESSION JO315240 VERSION JO315240.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 623) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 623) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..623 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 128 a 144 c 190 g 161 t ORIGIN 1 aattcccggg agtgggtgcc tgtgtgctgc tgcgggatgt gctgtgtgat ttattgcctg 61 aggtggtgtg agccagtata gctcccagtt gcatcacctg caagcgagct gagatgacct 121 gcctggtgtg atgttctgga aggaggtgtg atgccagagc ctgtgaaaac gatctcattg 181 cctgaatcac atgccaggtg atggtatgag gcggctgatc tggaagtatt gcagggcgcc 241 catgtgcctg catgatcttg gaatgactaa cacgccccgt tgctgactca gacccaatca 301 tgagtctgag ctctgctggc gttgaccgct gcctttgaac acttcagagg tgcgcatcgt 361 tcactgcatg gcattgacta ctgaggcacc ttatgcaatc aaacaactca atcgcgagtg 421 tcagtctatg cgtgcagtgt gggtgtcatg tggggatcag cttctgcgga gtggcatgga 481 gacatgtggt ggcggctgca tcagcactgc cacgcgtctt tggtcgttgc ggtgctacag 541 ccatttgtag agtggttggc gctctggaag cttggaaaca caccttctgt agttgtttat 601 gcagaacatc acaccagagc aaa //