LOCUS       EF822552                 516 bp    DNA     linear   GSS 02-JUL-2007
DEFINITION  Mus musculus C57BL/6N IST11461B3HMF1 gene trap embryonic stem cell
            insertion site sequence tag (IST), genomic survey sequence.
ACCESSION   EF822552
VERSION     EF822552.1
KEYWORDS    GSS.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Hansen,G.M., Markesich,D., Zhu,Q., Richter,L.J., Abuin,A.,
            Sands,A., Finnell,R.H. and Zambrowicz,B.P.
  TITLE     Large-Scale Gene Trapping in C57BL/6N Mouse Embryonic Stem Cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 516)
  AUTHORS   Finnell,R.H. and McLeod,M.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2007) TIGM, 2121 W. Holcombe Blvd, Houston, TX
            77030, USA
COMMENT     Contact: Richard H. Finnell at Texas Institute for Genomic Medicine
            (TIGM)  Phone: 713-677-7429  Fax: 713-677-7790  Email:
            ncbigss@tigm.org  Website: www.tigm.org.
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Mus musculus"
                     /mol_type="genomic DNA"
                     /strain="C57BL/6N"
                     /db_xref="taxon:10090"
                     /clone="IST11461B3HMF1"
                     /sex="male"
                     /cell_line="Lex3.13 ES transgenic cell line harboring
                     insertion mutation"
                     /cell_type="embryonic stem cell"
                     /transgenic
                     /note="strain origin confirmed on the basis of 110 genetic
                     markers distributed across 19 autosomes and the X
                     chromosome (Charles River Laboratories); sequence tags are
                     derived from genomic sequence by inverse PCR (IPCR) and
                     represent the gene-trap vector insertion site; sequence
                     reads represent either the upstream (F) or downstream (R)
                     vector-genomic junction, amplified from circularized
                     genomic DNA"
     source          1..30
                     /organism="Gene trapping vector VICTR76"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:447635"
     repeat_region   <1..30
                     /rpt_type=long_terminal_repeat
BASE COUNT          145 a           97 c          121 g          123 t
ORIGIN      
        1 gattgactac ccgtcagcgg gggtctttca ntgcacannt acacattcag gcaaatgcac
       61 taagtagttt aattttacac taatttattt gtgtaggagg cagagtatac aagaagacaa
      121 cttaaggaag tctgtttttt ccttccnaca cgtggggcca ggggactgaa tccgtgtcac
      181 tgggcttgaa gctagtgctt gacatgctga cacatntnnt aagctctact tttatgaacn
      241 atcagcttta nctgggtggt ggcggcactc aggaggcana ngcaggcana tctctggttt
      301 taaggttagc ctgacctacn tacagagtga gttccangac agtcanagct gaaacanann
      361 ccctgtcang gacaggaaaa aggaanagag ccnngcatgg aagcagatgc ctatantccn
      421 agtatgaggg aagctaangc aagattataa cttcnaggtc nagctaggct tcatagtgag
      481 actgnnataa ggaaacatta attgcnacag ttaacg
//