LOCUS CK334876 458 bp mRNA linear EST 12-MAY-2010
DEFINITION H3118B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3118B07 5', mRNA sequence.
ACCESSION CK334876
VERSION CK334876.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 458)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3118 row: B column: 07
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..458
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3118B07-5"
/db_xref="taxon:10090"
/clone="H3118B07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 105 a 112 c 134 g 107 t
ORIGIN
1 gtggccggca ggtgagagga ggtgctgcaa gactctctgg tcgcagaatg aagagggttc
61 tggttctcct gctggcctta gcctttgggc acgctctaga gagaggccga gactatgaga
121 aggataaagt ttgcaatgaa ctcgccatgc tggggaaaga ggacttcaga tctttatcac
181 taatcctata tagcaggaag ttttccagta gcacatttga gcaggtcaac cagcttgtga
241 aggaagttgt ctccttgact gaggagtgct gtgctgaggg agctgacccc acctgctacg
301 acaccaggac ctcagagctg tctgttaagt cctgtgaaag tgatgctccc tttccggttc
361 accctggaac tcctgagtgt tgcaccaagg aggggctgga gcggaaactc tgcatggctg
421 ccctcagtca ccagccgcag gaatttccca cctacgtg
//