LOCUS       CK334624                 556 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3067E01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3067E01 5', mRNA sequence.
ACCESSION   CK334624
VERSION     CK334624.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3067  row: E  column: 01
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3067E01-5"
                     /db_xref="taxon:10090"
                     /clone="H3067E01"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          164 a          131 c          141 g          120 t
ORIGIN      
        1 gtttgccgtc agaacgcagg tgttgtgaaa accaccgcta attcaaagca aaaatgggaa
       61 gggaaaagac tcacatcaac atcgtcgtaa tcggacacgt agattccggc aagtccacca
      121 caaccggcca cctgatctac aaatgtggtg gaatcgacaa gcgaaccatc gaaaagtttg
      181 agaaggaggc tgctgagatg ggaaagggct ccttcaagta cgcctgggtc ttagacaaac
      241 tgaaagctga gcgtgagcgt ggtatcacta ttgacatctc cctgtggaaa ttcgagacca
      301 gcaaatacta tgtgaccatc attgatgccc caggacacag agacttcatc aaaaacatga
      361 ttacaggcac atcccaggct gactgtgctg tcctgattgt tgctgctggt gttggtgaat
      421 ttgaagctgg tatctccaag aacgggcaga cccgcgagca tgctcttctg gcttacaccc
      481 tgggtgtgaa acagctgatt gttggtgtca acaaaatgga ttccaccgag ccaccataca
      541 gtcagaagag atacga
//