LOCUS CK334603 711 bp mRNA linear EST 12-MAY-2010
DEFINITION H3063D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3063D03 3', mRNA sequence.
ACCESSION CK334603
VERSION CK334603.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 711)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3063 row: D column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..711
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3063D03-3"
/db_xref="taxon:10090"
/clone="H3063D03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 219 a 156 c 151 g 185 t
ORIGIN
1 aaaccacaag ctcatttgaa gattcaggga gatcctcaaa ccagtccttg agaattccaa
61 gaggtcttat ttggtatatg aggcccaatt tataaaaaca aagaatagga aggtaaaagg
121 caaattgcag aaagactctg gctttgcttg ggaagtagac tacggggctc tgactcccca
181 agtaagtaag actttaggca tctctggata cttttcaatt actgagtcta gcaggctaac
241 tgttaagctt catatacttg acttcactca ccctccctca tgggttgatg ttctgcatcc
301 tgtaggcagg gaacttggag gagtaatgtc agacaccaag actccatcag ataccagaat
361 gcagacactg gaaattctgt caccccagta aactaacact gagcgctcac gatccaggtc
421 ctgtgttttt ctgaaaactg cactcagcaa caacaacaat atatgtaaag acagttcata
481 gaaactgaac aatgccaagc tccttattgg atgagaaacc cagtctacct tgcttacaga
541 agtacatcag tggtacagaa actagttcat ctgcatatac attggcaaat gattgtggat
601 gcaaagaagt gtgtgccgcg catttcttat ttgcacagaa ggacatgccc tccagggtga
661 tgttaagttt atagcaacag aggcctctcc tctttctggg acatctggag t
//