LOCUS       CK334574                 582 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3056B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3056B04 3', mRNA sequence.
ACCESSION   CK334574
VERSION     CK334574.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3056  row: B  column: 04
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3056B04-3"
                     /db_xref="taxon:10090"
                     /clone="H3056B04"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          111 a          154 c          175 g          142 t
ORIGIN      
        1 gacagtctca agtttttatt cagtgggtct ctgtgtctag aagaaggtgt tgggcctctt
       61 ggtggtgaag cgtggcttgt gctggcgccg caacacacgg tggggcagtg ggaatttgat
      121 cttggagtcg tggaactgct tgacagctgg ccggcggcac ttgccagctg caatctcttc
      181 caccttcatg atctggatgg agtgggcacg ggcacggtgt cgggcaccca tgtctcggta
      241 gcactgtgtg actgcaccag cagtggtcag gtccctgtac tcccgataca tgttgtgtgt
      301 gccactgcgg gagtcatagc gcagccagat gccaaagttc ttcacacgca ggggtgactt
      361 ctcaaacacc tgtccgcagt acacaatttc cccggatgac ttcttcatct tcttcagctg
      421 tgacacaaag taccagaagc gggacttggc caccacatgg ttgggtgcaa agattcgcat
      481 acggtacagt ggtggtgtgt ggcattttgg ggttggcaag cagcgcccca ccaccttgta
      541 ctcccgaagc gtgcccgagg ccttcatggc actgcgcact cg
//