LOCUS       CK334463                 585 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3032E12-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3032E12 5', mRNA sequence.
ACCESSION   CK334463
VERSION     CK334463.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3032  row: E  column: 12
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3032E12-5"
                     /db_xref="taxon:10090"
                     /clone="H3032E12"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          185 a          131 c          154 g          115 t
ORIGIN      
        1 ggaaccatgg aggctgttcc agagaagaaa aagaaggttg ccactgtgcc aggaaccctt
       61 aagaaaaagg ttcctgctgg gccaaaaact ctcaagaaaa aggttcccgc tgtgccagaa
      121 accctcaaga aaaagcgaag gaatttcgca gagttgaagg tgaagcgcct gaggaagaag
      181 tttgccctga agacacttcg aaaggcaagg aggaagctca tctatgagaa ggcaaagcac
      241 tatcacaagg agtacaggca gatgtaccgc actgagattc ggatggccag gatggcaagg
      301 aaagctggca acttctatgt gcccgcagaa ccaaagctgg cctttgtcat cagaattcga
      361 ggtatcaatg gagtaagccc aaaggttcgt aaggttctgc agctccttcg tcttcgacag
      421 atcttcaatg gcacctttgt taagctcaac aaggcttcaa ttaacatgct gcggattgtg
      481 gagccataca ttgcatgggg gtaccccaac ctgaagtcag taaacgagct catctacaag
      541 cgaggctacg gcaaaatcaa caagaagcgg attgccttga cagat
//