LOCUS BG088854 518 bp mRNA linear EST 12-MAY-2010
DEFINITION H3158D10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3158D10 5', mRNA sequence.
ACCESSION BG088854
VERSION BG088854.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 518)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG088854.1.
Other_ESTs: H3158D10-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3158 row: D column: 10
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..518
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3158D10-5"
/db_xref="taxon:10090"
/clone="H3158D10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 129 a 152 c 133 g 104 t
ORIGIN
1 agcagattcc cccggcccct cccaggccag actttgaggc ttcaagggaa aggctgcaga
61 agttgggtga gggcaacagc tccatcaccc gggaagaatt ctccaagatg aagcaggagc
121 tagaagcgga atacttggcc atctttaaga agactgttgc gatgcatgaa gtcttcctgc
181 agcgcctggc agcccatccc accctgcgtc gggaccacaa cttctccgtc ttcttggaat
241 acagccagga tgaggtagat gacttctttg agcacgagag gaccttccta gtagaatacc
301 acacccgaat ccgagacacc tgccagaggg ccgaccgtgt tatgcactcc cacaagtgcc
361 tggcagacaa ctatatccct atctctgctg cactgagcag tctcggaacc caggaagtca
421 accaactcaa gaggagcttc ctgaagctgg cagagctctt tgaacggctg agagtgagta
481 ctgccttctg cactcggtct tccctagcag cgccatag
//