LOCUS       BG087483                 607 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3140C07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3140C07 5', mRNA sequence.
ACCESSION   BG087483
VERSION     BG087483.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG087483.1.
            Other_ESTs: H3140C07-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3140  row: C  column: 07
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3140C07-5"
                     /db_xref="taxon:10090"
                     /clone="H3140C07"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          189 a          123 c          132 g          163 t
ORIGIN      
        1 atcaacaaga ggatctctgg gtctgaaaag gagccccagt ttaagttcat ctacttcaac
       61 cacatgaatt tagcagagaa aagtacaatt cacatgagga aaacgcccag tgtgtcactc
      121 acatccgtcc acccagatct aatgaagata ttaggggaca tcaacagtga ctttaccaga
      181 gcagatgaag atgaagaaat cattgtgaag gccatgagcg actactgggt tgttgggaag
      241 aagtctgacc agcgggagct gtacgtgatt ctcagtcaga agaatgcgaa cctgatcgag
      301 gtcaatgaag aggtgaagaa actgtgtgcc acgcagttca acaacatctt cttcttggat
      361 tgactccact ggctcagcag aacatcttta aaaacctcaa caacctttgt gtttaccatt
      421 acaacttact ggtcatgcta gtgactgggt tagggacaca gtaaagcaat acctgcttgg
      481 aaaaccacat ttctacttgg tctgatgatt ccctgaggtt tctagatgtt aaatatttat
      541 gtgaaattaa ttaaatgtag tcagctgaat accattccat tctgctgaca ttgtatgggt
      601 attttac
//