LOCUS       BG087477                 543 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3140B11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3140B11 5', mRNA sequence.
ACCESSION   BG087477
VERSION     BG087477.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG087477.1.
            Other_ESTs: H3140B11-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3140  row: B  column: 11
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3140B11-5"
                     /db_xref="taxon:10090"
                     /clone="H3140B11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          153 a           94 c          147 g          149 t
ORIGIN      
        1 gcccgggtga atcagtcctg ggttttaaag gatgattttt aggagttgtt gtgaaaacag
       61 cctaagaaag tacattgccc caggaaaatg tcaccaccga gtgccatgtt tctctgcttt
      121 tgtccctttg gtctgcattg tgtaaataca gggtcactct tctgaggagg taagcaaaac
      181 attaagtaaa aagtctctct cagtcttact gtgagagtca gtgaaatgaa gtctggcagt
      241 ataaaataaa atgcacaata aatgttagct gccatcagta ttgtgtcctc atggtgagtt
      301 tatgtctaag cacatcgttc atatgaggtg atttcctgtg gggaatcctt aacgagagaa
      361 gctgaatagg caaagagggg gtaacctaaa tgagttgcag ggggatttcc cttgggtgtg
      421 tcaagtcaga tggaggactg agggttaagt gttgattgac atgtaattgc accaatagat
      481 gacaggcatc agagacagag tgggtgggac ccaagggttg ccatccattc acctgtggga
      541 agc
//