LOCUS BG087361 587 bp mRNA linear EST 12-MAY-2010
DEFINITION H3138F11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3138F11 5', mRNA sequence.
ACCESSION BG087361
VERSION BG087361.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 587)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG087361.1.
Other_ESTs: H3138F11-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3138 row: F column: 11
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..587
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3138F11-5"
/db_xref="taxon:10090"
/clone="H3138F11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 135 a 162 c 181 g 109 t
ORIGIN
1 cccagagggg ccctgggaag agccgctgca gctccggaaa ctgcaagagc tttctatcca
61 aggagacagc gagaaccaag tgctggtgct ggtggagcgg aggaggacac tgtacatcca
121 gggtgagcgg cggctggact tcatggcttg gctaggggtc atccagaaag cagcagccag
181 cttgggagac acactatcag aacaacagct tggggactcg gacatcccag tgattgtgta
241 ccgctgtgtg gactacatca cgcagtgtgg cttgacctca gagggtatct atcgaaagtg
301 tggtcagacc tccaagactc agagactgct agacagcctc cggcaggacg cacgctctgt
361 gcacctaaag gagggagaac agcacgtgga cgacgtctcc tctgcactca aacgcttcct
421 cagggacctg cccgatgggc tcttcacgcg tgcgcagcgc ctggcctggc tggaggcctc
481 tgagatcgag gatgaggaag aaaagatctc caggtatcga gagctcttgg tgcatctgcc
541 ccctgtcaac cgggccactg tgaaggccct tatcagccat ctgtact
//