LOCUS BG086572 671 bp mRNA linear EST 12-MAY-2010
DEFINITION H3127H07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3127H07 5', mRNA sequence.
ACCESSION BG086572
VERSION BG086572.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 671)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086572.1.
Other_ESTs: H3127H07-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3127 row: H column: 07
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..671
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3127H07-5"
/db_xref="taxon:10090"
/clone="H3127H07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 183 a 226 c 161 g 101 t
ORIGIN
1 ccacccccag atgtcagcca tagaaaccat gacggagaag ctggagagct ttgcagccat
61 gaatgccgac tcaggcagct ccctgcagcc cctgcctcac cacccgttca acttccgctc
121 cccaccccca acgctctcgg atcccatcct caggaagggg aaggagagat acacgtgcag
181 gtactgtggc aagatcttcc ccagatctgc aaatctcaca agacatctga ggacacacac
241 aggggagcag ccatacaggt gcaagtactg tgaccggtca ttcagcatct cctccaacct
301 ccagcggcac gtgaggaaca tccacaacaa agagaagccg ttcaagtgcc atctgtgcaa
361 ccgctgcttc gggcagcaga ccaacctaga ccggcacctg aagaagcacg aacacgaggg
421 cgcaccagtg agccagcact ccggggtgct cacgaaccac ctgggcacca gcgcctcctc
481 ccccacctcc gagtcggaca accatgcact tttagatgag aaggaagatt cttacttctc
541 cgagatccga aacttcatcg ccaacagcga gatgaaccag gcatccactc gaatggacaa
601 acggcctgag atccaagacc tggacagcaa cccaccgtgt ccaggctcag ccagtgcaaa
661 gccagaggac g
//