LOCUS BG086050 730 bp mRNA linear EST 12-MAY-2010
DEFINITION H3121B10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3121B10 5', mRNA sequence.
ACCESSION BG086050
VERSION BG086050.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 730)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG086050.1.
Other_ESTs: H3121B10-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3121 row: B column: 10
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..730
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3121B10-5"
/db_xref="taxon:10090"
/clone="H3121B10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 184 a 181 c 176 g 189 t
ORIGIN
1 attctaccct ggcaagatgg gaaacaggta catgtggtca gtactctgac tacctacatg
61 atccccaaac cagaagaaat taacttgctc actggggagt ctgctacaca gcaacagata
121 gaagccgaga agaagccatc aaacgccctg gatgaacccg tctctcactg gcgaccaagg
181 ctgaccctga atgtgatggt ggatgacttt gtctttgatg gttcctccct gcctgcagat
241 gtgcatcggt acatgaagat gattcagctt gggaagacag tgcactacct ccccatcctg
301 ttcattgacc agctgagcaa ccgggtgaaa gacctgatgg tgataaaccg atctaccacc
361 gagctgccac tcactgtgtc ctacgacaag atctcattgg ggcggctgcg cttctggatc
421 cacatgcagg atgccgtgta ctccctgcag cagttcgggt tttcagaaaa agatgcagat
481 gaattgaagg gaattttcgt agacaccaac ttatatctct tagcattgac cttctttgtt
541 gcagcctttc accttctttt tgatttcctg gcatttaaaa gtgacatcag tttctggaag
601 aaaaagaaga gcatgattgg aatgtctacc aaagcagtgc tctggcgttg cttcagcact
661 gtggtcatct tcctcttcct gctggatgaa cagacaagcc tgctggtgct gatccctgca
721 ggtgttggag
//