LOCUS       BG085654                 681 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3115H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3115H10 5', mRNA sequence.
ACCESSION   BG085654
VERSION     BG085654.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG085654.1.
            Other_ESTs: H3115H10-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3115  row: H  column: 10
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3115H10-5"
                     /db_xref="taxon:10090"
                     /clone="H3115H10"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          183 a          139 c          179 g          180 t
ORIGIN      
        1 ccatgttctc gcgggcgagc gttgtcgggc tgtcggcctg cgccgtgcag ccgcaatgga
       61 tccaagttcg aaacaaggca actccgaaag atattaccag gagactgaag tccatcaaaa
      121 acatccagaa aattaccaag tctatgaaga tggtggcagc tgcaaagtat gcccgggctg
      181 agcgggagct gaagcctgcc cgagtgtatg ggacaggttc tttggctctg tatgagaagg
      241 ctgatattaa ggcacctgag gacaagaaga agcacctcat tattggtgtg tcctcagata
      301 gagggctttg tggtgctatt cactcctcag tggctaaaca gatgaagaat gaagtggctg
      361 ccctcacagc agctgggaaa gaagttatga ttgttggagt tggtgaaaaa atcaagggca
      421 tactttatag gactcattct gatcagtttt tggtgtcatt caaagatgtg ggacggaagc
      481 ctcctacttt tggagatgca tcagtcattg cccttgagtt gttaaattct ggatatgaat
      541 ttgatgaagg ctctatcatt tttaatcagt tcaagtctgt tatctcctac aagacagaag
      601 agaagcccat cttctctctg aataccattg cgactgctga gaccatgagc atctatgatg
      661 acattgatgc tgatgtgctg c
//