LOCUS       BG085068                 499 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3108C05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3108C05 5', mRNA sequence.
ACCESSION   BG085068
VERSION     BG085068.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3108C05-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3108  row: C  column: 05
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3108C05-5"
                     /db_xref="taxon:10090"
                     /clone="H3108C05"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          136 a           82 c          133 g          148 t
ORIGIN      
        1 cctttcctcc ccaacttgtt tcttggtcat gatgtttgtg caggaataga aaccccgact
       61 aagacaaatt ggtaccagtg gagtggggta ttcctgtgac aacctgacca tgttttgggg
      121 aggactgtgg aaggactttg gaactttggg cttgaagatc catccattgt taagacctct
      181 gtcggatgtt gtgtaggagc ttggaagata atgttgagaa cactgcagaa gttggagctc
      241 tggtgtgtga aatttcagag ggaaaattaa agactctttt cagggccatt tctgttttga
      301 ttgtgaagat tctgtagttc tggttagctg gggctgaaga atcagctgtg attaacaaga
      361 taccagaact actaaagcaa aaactttgca ttactgggac tattgatgct ggttagctgg
      421 agctaagaaa ttagcggtga ttaagaagag accagcatca ttgaggtgac atcttctggg
      481 aagtattttc tgaaagcac
//