LOCUS BG082616 538 bp mRNA linear EST 12-MAY-2010
DEFINITION H3078B04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3078B04 5', mRNA sequence.
ACCESSION BG082616
VERSION BG082616.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 538)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG082616.1.
Other_ESTs: H3078B04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3078 row: B column: 04
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..538
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3078B04-5"
/db_xref="taxon:10090"
/clone="H3078B04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 142 a 142 c 122 g 132 t
ORIGIN
1 caccgatttg gataaactga tcagcaaacc taaggggatc ctcctgtctc tacctcctca
61 gtgctggaat tacataaatg cttatcccca gcttttttac agggggtgtg gagcactaac
121 taaggttcct aagcttgcac agtgtgaact gagccatctc cccagcccca cttccctaac
181 ctctgagtga gtcaggcact ctgatttctc ttgttaacgt cctgaaatgt cttaaaacgt
241 cataggatac agtcaggtca tgcactgttc actcacaggt ttgctccatg tgcatgctaa
301 gtacattatc tatactgagc tacacccctg ccctaagagt gccatttaaa atgctaatag
361 cagaagacct tagcattcag cataattaaa agaagggaac caagccgggc gtggtggcgc
421 acgcctttaa tcccagcact cgggaggcag aggcaggcgg atttctgagt tcgaggccag
481 cctggtctac aaagtgagct ccaggacagc cagggctata cagagaaacc ctgtctcg
//