LOCUS BG081136 584 bp mRNA linear EST 12-MAY-2010
DEFINITION H3061E06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3061E06 5', mRNA sequence.
ACCESSION BG081136
VERSION BG081136.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 584)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG081136.1.
Other_ESTs: H3061E06-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3061 row: E column: 06
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..584
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3061E06-5"
/db_xref="taxon:10090"
/clone="H3061E06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 146 a 173 c 93 g 172 t
ORIGIN
1 ggcaaggcta agatgccact aatattcact ggaccccaac atcccacagc acctcccaca
61 gatctcagtc catcttacac tctctcgcct tccacgcctg tcagcagaag cacctactac
121 accatgtggg cttctggtta tgtgtcttct ctggcttcgt cttgggggtg gggacttttg
181 tcatggcatg ctttgccatg tttaatcctc ttagctacac taagagactc tactacttat
241 ctttttatgg ctgaaaaaat ctgtatcatt ctcatcagta tataaacagg cccccatgcc
301 atcttacaaa aaaacaaaca aacaaaaaaa aaaccccaaa acaaacccaa ccccttcatg
361 gattgcactc tccttcccag tccacacccc tttcattccc tgaatccttg gaaaagccct
421 cacttttctc tcttccaaag tacctctatg ctttaatctc tgccagttct gcattaaaca
481 ccacagctgc atctttttac ctcatgtctt ttgcatgtgt ctgagttgga tgtttcaccc
541 aactgagtgg tgtctgtgtt ggtgctggcc agcctagagc atct
//