LOCUS BG080547 564 bp mRNA linear EST 12-MAY-2010
DEFINITION H3054H08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3054H08 5', mRNA sequence.
ACCESSION BG080547
VERSION BG080547.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 564)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG080547.1.
Other_ESTs: H3054H08-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3054 row: H column: 08
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..564
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3054H08-5"
/db_xref="taxon:10090"
/clone="H3054H08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 158 a 165 c 139 g 102 t
ORIGIN
1 ctcgcgccac cagcgcaatg ggctcggagg cagctcagct gctggaggct gctgacttcg
61 ccgctcacaa acaccgacag cagcgacgaa aagatcctga agggactccc tacattaatc
121 accccatcgg tgtagcccgg atcctaaccc atgaggctgg aatcactgat attgtggtgt
181 tacaggccgc cctgctccat gacacagtag aagacacaga tactaccctg gatgaggtag
241 agctgcactt tggagcacag gttcgacgct tggtggagga ggtaacagat gacaagactc
301 tgcccaaact ggaaagaaag cggcaacagg tggagcaggc accacacagc agcccagggg
361 ccaaactggt gaagctggca gacaagctat acaatctgag ggacctgaat cgctgcaccc
421 ctacagtgtt ctcaacatta tcttccaagc tcctacatga catccgacag agctcttaac
481 aacggatgga tcttcaagcc caaagttcca aagtccttcc acagtcctcc ccaaacatgg
541 tcagattgtc acaggaatac ccca
//