LOCUS BG079649 687 bp mRNA linear EST 12-MAY-2010
DEFINITION H3043A04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3043A04 5', mRNA sequence.
ACCESSION BG079649
VERSION BG079649.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 687)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG079649.1.
Other_ESTs: H3043A04-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3043 row: A column: 04
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..687
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3043A04-5"
/db_xref="taxon:10090"
/clone="H3043A04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 173 a 186 c 145 g 183 t
ORIGIN
1 ttgggattca gcctgcctcc tggtactacc cagttagatc agaaccggtt cactactata
61 gaagataagc tatgcagagt cccagccagt cccagttgaa gagaggtatt gagggaaata
121 tggctagact tgccaccagg ggcagctggc tagcaagcca ctcataggcc cctgactgct
181 ggtctctctt ggtggcagat ctttcctacc acttctttct gtgggattct tacagattct
241 ccatccaacc ctacctaacc gcctcagcag tcttaaaaat gacacacacc gagtttgcga
301 ttgcttcagg gacgagaaca cagtgactcc ctggatttgc tgctacaatt ctttactctc
361 tgttcttgtc gcagaaccta ttagtggctt tctttagccc taaggcaaaa ataaacctgc
421 catgaaagta gatcattgac cttaattgta aggtgactga ctaggaagaa ggacaaaggt
481 ggcagctgct aacggccatc gtctctactg tcatccttcc catgcctctg atctgctcac
541 agcacatttt acatgcatgt tgccttcctg acttctaggg attctggtgg gactgcaaat
601 caactgtcct gctctgtgcc cacacaacta accaagcttg gacttctgca tagcctatcc
661 catagtgaac caatccagag atgacac
//