LOCUS BG079136 666 bp mRNA linear EST 12-MAY-2010
DEFINITION H3036B11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3036B11 5', mRNA sequence.
ACCESSION BG079136
VERSION BG079136.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 666)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG079136.1.
Other_ESTs: H3036B11-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3036 row: B column: 11
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..666
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3036B11-5"
/db_xref="taxon:10090"
/clone="H3036B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 136 a 213 c 191 g 126 t
ORIGIN
1 gagaacccct gctcagcagc tctgagaatg gcactggagg cgcagagatg gctccagctg
61 atgccaggac agagcggctc ctcaatgacc tcctgccctt ctgcagacca gtcccagagg
121 gccagcttgc tgccacagcc cttcagaaag tactgtccct gaagcagatc ctgctaagca
181 ctgaggaaga cagtggagcg gggcctcccc gcgatgggga tggggtgcct ggtggtaggg
241 cccccggccc agtgcacacc caggagattg aggaaaactt gcttagctta gaggtggcca
301 tcagacaact ggaggagttg gaagaggaat tttgtcgcct aagacccctc ctgtcccagc
361 ttggggggac tctgtccccc aacctggctg cacctgaacg ctctgctcgg acaggccttt
421 catgaagaga agagtggggg agaggatgca ggggggccca cccccacccc cacagctgcc
481 acagcatctc acaccccaga ggcctggagc agagggagat tggctgaact tgatagcaga
541 cctgctcggg tccctgcctc ccactctggc ctctgccttt ctccctcctg ccttctgctt
601 ggggaactca gggcttcact cccagagcac ccccttaatc cccacttttc aggccgtctc
661 agtatt
//