LOCUS       BG077966                 598 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3021B02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3021B02 5', mRNA sequence.
ACCESSION   BG077966
VERSION     BG077966.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077966.1.
            Other_ESTs: H3021B02-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3021  row: B  column: 02
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3021B02-5"
                     /db_xref="taxon:10090"
                     /clone="H3021B02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          174 a          153 c          164 g          107 t
ORIGIN      
        1 ctttgcgaat tctgtggaca atgcccgcat cgtcttgcag atcgacaatg cccgccttgc
       61 cgccgatgac tttagagtca agtatgagac agaactagcc atgcgccagt ctgtggagag
      121 cgacatccat ggactccgca aggtggtaga tgacaccaac atcacaaggc tgcagctgga
      181 gacagaaatc gaggcactca aggaagaact tctgttcatg aagaagaatc atgaagagga
      241 agtccaaggt ctggaagccc agattgccag ctctggattg actgtggaag tggatgcccc
      301 caaatctcag gacctcagta agatcatggc ggacatccgc gcccagtatg aagcgctggc
      361 tcagaagaac cgcgaggaac tggacaagta ctggtctcag cagattgagg agagtaccac
      421 agttgtcacc accaagtctg ccgaaatcag ggacgctgag accacactca cggagctgag
      481 acgcaccctc cagaccttgg agattgactt ggactccatg aaaaaccaga acatcaactt
      541 ggagaacagc ctcggggatg tggaggcccg atacaaggca cagatggagc agctcaat
//