LOCUS       BG077710                 614 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3018G08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3018G08 5', mRNA sequence.
ACCESSION   BG077710
VERSION     BG077710.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077710.1.
            Other_ESTs: H3018G08-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3018  row: G  column: 08
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3018G08-5"
                     /db_xref="taxon:10090"
                     /clone="H3018G08"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          132 a          179 c          183 g          120 t
ORIGIN      
        1 gtcagcgctg gagcttccgc aggcgccatg gaaaaggtgc tggtcacagg tggggctggc
       61 tacatcggca gccacacggt attggagctg ctggaggcag gctactcacc tgtggtcatt
      121 gacaacttcc ataacgccat tcgtggagaa gactccatgc ctgagagcct gcgccgggtc
      181 caggagttga caggccgctc tgtggagttt gaggagatgg acatcttgga ccaggcagcc
      241 ctacagcacc tctttaagaa gcacagcttt aaggccgtca tccactttgc tggcctcaag
      301 gctgtgggcg agtcagtgca gaagcctctg gactactata gagttaactt aacagggacc
      361 atccagcttc tagagatcat gagggcccac ggagtgaaga acctggtgtt cagcagctca
      421 gccaccgtgt acgggaaccc ccagtacctg cctctggatg aggcccaccc cacggggggc
      481 tgtaccaacc cctacggcaa gtccaagttc ttcatcgagg agatgatccg ggacctgtgc
      541 cgggcagaca cggcctggaa cgccgtgctg cttcggtact tcaatcccat aggcgcccac
      601 gcctctgggc gcat
//