LOCUS BG076134 715 bp mRNA linear EST 26-JAN-2001
DEFINITION H3156A08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3156A08 3', mRNA sequence.
ACCESSION BG076134
VERSION BG076134.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 715)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3156A08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3156 row: A column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..715
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3156A08-3"
/db_xref="taxon:10090"
/clone="H3156A08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 285 a 128 c 122 g 180 t
ORIGIN
1 cgtatttcta cataaacaac tctattacaa caaagccagg taaacccaac ggacacaggt
61 ctttaggaaa acaattttcc cttacatata aaagggtcat tttctatggc aacagataca
121 gaagaaggcg ttagacacat aggagaaggg ctgacagaca gtgatcatgg gaacacgcct
181 ttcttagtct ttaatgaatt aagaaaataa aatttaaagt ccataatagt atatttagta
241 aagtcaaatt aatatacata gatcaaatat tgttgctttc aaagaatata ccttctacaa
301 cttcctgggt atcttattaa aactaaaaat ttattaggtc tcaacaaaat gttaatgaac
361 tccgaaatgc acaagttgta caagccatac cctccttgac aacaggcgat gaagcagagt
421 ctgtaacaaa gttaaaaaca acagcaacaa agacaaacga tcaggaagtc taaaagtata
481 atttcaactc tctcctcagt aaaagataaa ctccagtttg aagggatgag ttatttatga
541 caatcacatc atatataaac atttatggga tacagatata ctcagaggaa aggtcacagg
601 cctacagcaa gagttaccct gtctctgtgc aagaagtcag catggcctgt aacatggaaa
661 gctggagaaa tgccaaggga aaaacaacaa tcataaaatg ataaatctgg aaata
//