LOCUS BG076079 691 bp mRNA linear EST 12-MAY-2010
DEFINITION H3155D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3155D07 3', mRNA sequence.
ACCESSION BG076079
VERSION BG076079.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 691)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG076079.1.
Other_ESTs: H3155D07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3155 row: D column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..691
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3155D07-3"
/db_xref="taxon:10090"
/clone="H3155D07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 278 a 119 c 108 g 186 t
ORIGIN
1 gccgcaatat atatatttta attcaaagtg aatcaacagt aatacaccat aaattcttat
61 tttgacactc accaaaatag tcacctggaa aacccgcttt ttgtgacaaa gtacagaagg
121 cttggtcaca tttaaatcac tgagaactag agagaaatac tatcgcaaac tgtaatagac
181 attacatcca taaaagtttc cccagtcctt attgtaatat tgcacagtgc aattgctaca
241 tggcaaacta gtgtagcata gaagtcaaag caaaaacaaa ccaaagaaag gagccacaag
301 agtaaaactg ttcaacagtt aatagttcaa actaagccat tgaatctatc attgggatcg
361 ttaaaatgaa tcttcctaca ccttgcagtg tatgatttaa cttttacaga acacaagcca
421 agtttaaaat cagcagtaga gatattaaaa tgaaaaggtt tgctaataga gtaacattaa
481 ataccctgaa ggaaaaaaac ctaaatatca aaataactga ttaaaaattc acttgcaaat
541 tagcacacga atatgcaact tggaaatcat gcagtgtttt atttaagaaa acataaaaca
601 aaactattaa aatagtttta gagggggtaa aatccaggtc ctctgccagg atgctaaaat
661 tagacttcag gggaattttg aagtcttcaa t
//