LOCUS BG075361 571 bp mRNA linear EST 12-MAY-2010
DEFINITION H3146C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3146C12 3', mRNA sequence.
ACCESSION BG075361
VERSION BG075361.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 571)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG075361.1.
Other_ESTs: H3146C12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3146 row: C column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..571
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3146C12-3"
/db_xref="taxon:10090"
/clone="H3146C12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 129 a 146 c 157 g 139 t
ORIGIN
1 cttttctttt cttttttttt tcctttttta caattttatt tataacaata tctgtgttat
61 ttagttgtaa aggaattcag caaaaattat taaaacgttc acgtccccaa aatggggctg
121 cccgcttgcc cttccttggt gtgccccgac tcttcctggc cctcaggggg atgggaggct
181 tgggggacag ttaccaaaga atgtgttcag ccctaggagc cacagagggg gcgctagaga
241 ggggagggcc tgtctggaag ggatactgag catggccatc tgagaggtgc ccctgaaagt
301 gggagctggg gtttggggtg acttccctca actaaaagac tccttcctca gctagagcca
361 aatcttccag acagggtcag cagaggactc cacctcccct gaactaaccc cagagagcca
421 aggaaaggaa agctgggaca cggagccccg ttgagtgtct tgcacaggcg tggtggggag
481 gccgtgcgcc agcttccagg ggtatgctcg gtagggaaca tgtggactcc ttcatacctt
541 tgagccacaa agccctcccc gccccctcaa g
//