LOCUS BG074837 626 bp mRNA linear EST 12-MAY-2010
DEFINITION H3140A02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3140A02 3', mRNA sequence.
ACCESSION BG074837
VERSION BG074837.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 626)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG074837.1.
Other_ESTs: H3140A02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3140 row: A column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..626
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3140A02-3"
/db_xref="taxon:10090"
/clone="H3140A02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 157 a 143 c 206 g 120 t
ORIGIN
1 cttatcaaaa actcacattg atttattaga atatgaaaaa ggttcagttt cctttgtaca
61 ggcggcagag cggcagcagc tgcggtggct ttgtacatgg ttgtcgatgt caccctgcct
121 ggcaactcct gcccaaaaca gcacttgcat ggagaggaga cagctgggcc atgcacccat
181 ggccccatcc cctgggaggg accctctgac tattattgca cgatcctggg gagggagtgc
241 ccaggggttg ggcagatacc catgggcttg gccccagcca tggtggcggt agggagtgtg
301 tgcctgtgag caaaggcctc tagggggtcc agtactttct actctacaga atgcaggaaa
361 gagagtggtc cagataaggg gtggtggtag aactggccaa cagaagtaaa agcaggccca
421 agagcaaagg aggtaccagg aggccccctg ctggtgagct tgagtcatga caggttgggc
481 cacatcagaa tcggtgctgt gggcaagggc ccccagcagc agaggttcca tttaagggac
541 ggtgttagac acgaggaagg acgaggggga aagggcagag ctctgactgg catgggggac
601 tcaaagggtc tgaaaacaag agtgcc
//