LOCUS BG072778 777 bp mRNA linear EST 12-MAY-2010
DEFINITION H3114H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3114H02 3', mRNA sequence.
ACCESSION BG072778
VERSION BG072778.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 777)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072778.1.
Other_ESTs: H3114H02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3114 row: H column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..777
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3114H02-3"
/db_xref="taxon:10090"
/clone="H3114H02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 241 a 157 c 189 g 190 t
ORIGIN
1 cactcatcac gtttattcag aagagaatac acccaatcct ctcatctctg gaaagttttg
61 ttcctagcca tcattaaaat caggacccct gttctcccct taaccccagg agaggaagct
121 tatagtcttt gtagatattc tgaacctctg atcacaaagg gttataaaat agtgaagggg
181 tttggactag gaaaggcaca gactgaaagg aatctgccag gggactgagg ccacagctcc
241 cccaggagtc agaggaaggg ggaagtcacg taatttagag aaggccaagg gcctcagagc
301 aagagtattc ctttgaagag ctcaggtcag gaggtttagc tcagctgggc ctggtagctc
361 aagctacttg gaagcctaaa gaagctgtgt gaagctctgg gttcaaaccc cagcaataaa
421 ataaaacaaa aaacagaggc tcagttcaaa gtctaattcc actgtcctgt ctaggttgaa
481 ccaatgagat ttctgtgtgg gcccaaggga tcagattgag ctgttgctct aaaacattga
541 gcagactcca gacagaaagt tgtcctggcc tctgtagaca gacattcagg aacaggtgaa
601 caagatagaa gttggtgact aaatccacag tgtcttgaag ttataaggga tgcatttttt
661 aaggatgtat tttaaaatag cacttttctg gctgtaccct tgcaagggag cacatgtcag
721 tgaatacctt ggtttccata aagtagggga agaagcagta aaaatagcaa agtttcc
//