LOCUS BG072397 822 bp mRNA linear EST 12-MAY-2010
DEFINITION H3110A11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3110A11 3', mRNA sequence.
ACCESSION BG072397
VERSION BG072397.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 822)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072397.1.
Other_ESTs: H3110A11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3110 row: A column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..822
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3110A11-3"
/db_xref="taxon:10090"
/clone="H3110A11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 247 a 206 c 167 g 202 t
ORIGIN
1 gctttttgag acagggtttc tctgtgtagt cctggctgtc ctggaattca ctttgtagac
61 caggctggcc tcgaactcag aaatccacct gcctctgcct cccaagtact gggattaaag
121 gcgagctcca ccacgcccag ccatatacaa atttcttgga taagaagggg tttcaagtaa
181 aaaaagttta agaagtcatg aaccaggagg ctggaggaat accctggtcg ttaggagcac
241 tggctgctct tccagaggcc ctagatttga ttcctagcac ccacatggaa gcttataact
301 ttaactacaa ttccaggaat ctgacaccct cttctggcct ttgcaggcac cagggacaca
361 tgatatacag acatacaaac aagtaaaaca ctctccacac acgaacttaa aaacaaacaa
421 acaaaatctt tttaaaaagt catgaatcag agtgttccct tttgggagta accccaaaga
481 tccctttctt gaaatctgac aacttaaggc tggaaagatg gcagagacag gcaggcctct
541 ggggcacact gaccgcctgc ttaggtatat ctatgaatct atgagttcca ggttcagtga
601 cagactctgt ctcaagaaaa ttaggttgag agtaatcaag gaagacacct aatgtcatgt
661 acccacatag acaaataaaa cacggtgtgc ctaaactccc cactgaaaaa cttgccaccc
721 tgaataggag tctcaacttc ctcaggccat cagacttcct cagactgcca gttttttgta
781 gagaccctct gactagcact tccacactca gttatttgtg gc
//