LOCUS       BG072374                 593 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3109G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3109G11 3', mRNA sequence.
ACCESSION   BG072374
VERSION     BG072374.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG072374.1.
            Other_ESTs: H3109G11-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3109  row: G  column: 11
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3109G11-3"
                     /db_xref="taxon:10090"
                     /clone="H3109G11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          135 a          148 c          148 g          162 t
ORIGIN      
        1 ctagtgtctg gcttttattg gttgcttgtt ttctcaaacc cagcattaaa aaaaagtcac
       61 ctgaggtggt agttttaggc agaggtggag atggggatag aacaggtcac ttggggtagg
      121 atgcaaacaa catgacaggt ccttcacatg tagtaccagg ccaagagtcc agctaccaag
      181 gccacactgg cagccagaat gagctgcagg ctaggcttcc tcagcacagc cacggttgtc
      241 tggaaggggc agttgctgcc tcctagtgta cctttgtctg gctgagcagc ataaaagggg
      301 catttacgta tatctccctt cccatcatgt accgggagcc catcctccag ggtttctctt
      361 gctagcatgg agccagcctg gtccagttca ctgaatatct gcatgttata ttcaaaggct
      421 ttattggcct cctccacaat cctctctttg gtcttcaaat tcaggtccaa ggcattcatt
      481 ctagcgcggt agaactgctt gaattgctgg gcattgtcca catgctcaaa caggtagaat
      541 tgggtccctt ccccagtgct gggaagtttt agtgccctct gggcaacctt ctt
//