LOCUS       BG072003                 537 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3105F02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3105F02 3', mRNA sequence.
ACCESSION   BG072003
VERSION     BG072003.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Other_ESTs: H3105F02-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3105  row: F  column: 02
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3105F02-3"
                     /db_xref="taxon:10090"
                     /clone="H3105F02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          116 a           94 c          125 g          201 t
ORIGIN      
        1 ggaagctgtc ttatgtgagg atgttttgct aaagcagaca cgtaaaaaga cgttttgctg
       61 agaacagaca catggtgctt ttctgggcgc tgtgtgagag tgcacataat gttatgccag
      121 agtggacgtg taatgttgag aaagaattta aattgaaccc cacagatagt aagaggatat
      181 gcttgcatta actaaccttg caacactttg gggntcttct ctgatgatgt tcttgcattg
      241 gtacaccttg caacacttaa ctgttttttt tttggttttg gttttggttt gttttttgtt
      301 ttttgtttgt ttgtttgttt gtttgtttgt ttgtttgttt tggctgatct tcgcttgcca
      361 tgacttcata gtgagaaagc agcaaagacc ttcttgaggt attctggctg cttctggctg
      421 cttctgcaag gctccactgc tcttcaatac aggatttctc tgagtagttc taggattcac
      481 tctgtatatt tggctgtcct caaactcaga gatccaccta cttctgcgga tagtcga
//